创面修复肉芽组织血管生成中周细胞的观测.doc

周细胞在创面修复早期血管生成中的分布与意义 孙慧勤, 冯一梅2章容1王明科2郭徳玉1 阎国和2邹仲敏2 （第三军医大学：1西南医院病理学研究所,2复合伤研究所,重庆400038）[摘要] 目的：观测及研究周细胞在创面修复肉芽组织血管生成过程中出现、分布规律与意义。方法：采用常规免疫组化、双重免疫组化染色，电镜技术及形态定量分析方法观测研究小鼠皮肤全层切割伤模型(1-9d)，特别是早期1、3、5、7d创面修复肉芽组织内血管生成过程中，周细胞的出现、分布规律。离体采用TGFβ1诱导分化的周细胞前体细胞10T1/2模型，观察其在细胞外基质胶立体培养的变化。结果：周细胞见于肉芽组织血管生成早期(1-7天)，且周细胞阳性染色的细胞数较内皮细胞多。肉芽组织内毛细血管和周细胞的数量在伤后持续增加，直到伤后5天达峰值。周细胞在肉芽组织中还可形成细胞条索状、腔样结构；离体培养的周细胞在细胞外基质胶内能够形成管腔样结构。结论：周细胞参与了肉芽组织早期血管生成过程并可形成腔样结构，可能在血管生成中具有主导或引导作用。 [关键词] 肉芽组织 血管生成 周细胞 The role of pericytes in the early angiogenesis of wound healing SUN Huiqin1, FENG Yimei2, ZHANG Rong1, WANG Mingke2,GUO Deyu1 ,YAN Guohe2 ZOU Zhongmin2 (1Institute of Pathology, Southwest Hospital, 2Institute of Combined Injury, State Key Laboratory of Trauma, Burns and Combined Injury, Third Military Medical University, Chongqing 400038, China) Abstract Objective To study the pattern of pericyte presence and distribution in granulation tissue of wound repair and its significance. Methods Granulation tissue samples were collected from mice suffered from full-thickness skin excision on 1, 3, 5, 7 ,9day after injury. Single and double immunohistochemical staining, electron microscopy, quantitative analysis of morphology were applied to study the pericyte presence and distribution during angiogenesis of granulation tissue. Meanwhile, in vitro TGFβ1-induced differentiation of 10T1/2 cell, a progenitor of pericyte, was assessed in 3-dimensional ECM gel. Results Pericytes appeared in the early stage of wound repair, the number of pericytes positive staining was higher than of endothelial cells at the new microvessels. The number of capillaries and pericytes in granulation kept increasing till 5 day after the injury. The pericytes stimulated with TGFβ1 were able to form cell cords and clumps in vivo and cell cord- or lumina-like structure in ECM gel in vitro. Conclusion Pericytes participate the early angiogenesis in the wound healing and the formed cell cord or lumina structure may play a c