Chapter1StructureandFunctionofProteins题材.ppt

Isoelectric focusing （IEF） 等电点聚焦 Two-dimensional electrophoresis 蛋白质组学研究的重要技术之一 4. chromatography 层析(chromatography) To separate proteins by different charge, size, binding affinity, and solubility 以由不同的电荷，大小，结合亲和力，和溶解度分离蛋白质 gel filtration chromatography Separates proteins by size分离蛋白的大小 Ion-exchange chromatography 离子交换色谱法 Separates proteins by charge Separates proteins by their binding specificities Affinity chromatography 亲和色谱法 分离蛋白的特异性结合 5. 超速离心 (Ultracentrifugation) Separation proteins on the basis of their densities massive particles always sediment faster particles sediment fa