Study of Enzyme Mechanisms.pptVIP

Study of Enzyme Mechanisms We have studied the mechanisms of peptide bond formation hydrolysis by an enzyme Why study mechanisms? Structure activity relationships → understand protein folding, etc Understand “superfamilies” Design enzyme inhibitors: Correct a metabolic imbalance Kill an organism: Herbicides/pesticides, antibiotics Diphtheria Toxin Corynebacterium diphtheriae ADP-ribosyltransferase EF + NAD+ → ADP-EF + nicotinamide Mechanism also present in other toxins Pertussis, E.Coli Binding to EF (eukaryotes) blocks translation Reaction Potential Mechanisms? Active Site with NAD+ Bo