不同来源基质细胞对骨髓瘤KM3 细胞增殖和凋亡的影响.docVIP

不同来源基质细胞对骨髓瘤KM3细胞增殖和凋亡的影响 高力，张曦，高蕾，陈幸华 （第三军医大学新桥医院血液科，重庆 400037） 摘 要：目的 观察人脐血基质细胞及骨髓瘤患者骨髓基质细胞对骨髓瘤KM3细胞生物学特性的作用。方法 检测与人脐血基质细胞及骨髓瘤患者骨髓基质细胞共培养的KM3细胞的增殖、细胞周期和凋亡率。结果 人脐血基质细胞比骨髓瘤患者骨髓基质细胞有更强的抑制KM细胞增殖的作用；共培养后骨髓瘤患者骨髓基质细胞使KM3细胞阻滞于G0/G1期，人脐血基质细胞使S期的KM3细胞比例增高；与人脐血基质细胞共培养后KM3细胞的凋亡率明显高于骨髓瘤患者骨髓基质细胞共培养组。结论 骨髓瘤基质细胞微环境抑制骨髓瘤细胞凋亡和死亡；而人脐血源基质细胞有更强的抑制骨髓瘤细胞增殖的作用。 关键词： 多发性骨髓瘤；人脐血基质细胞；骨髓基质细胞；增殖；凋亡 中图分类号：R75-351;R730.2;R733.71 文献标识码：A Effect of stromal cells derived from different origins on proliferation and apoptosis of KM3 cells GAO Li, ZHANG Xi, GAO Lei, CHEN Xing-hua (Department of Hematology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China ) Abstract: Objective To observe the effect of the human umbilical cord blood-derived stromal cells (hUCBDSCs) and multiple myeloma bone marrow stromal cells (MM BMSCs) on the biological characteristics of KM3 cells. Methods After KM3 cells were co-cultured with hUCBDSCs or MM BMSCs, the proliferation, cell cycle and the apoptotic rate were determined by growth curve plotting and flow cytometry respectively. Real-time Q-PCR was used to measure the mRNA expressions of Bcl-2 and Bax. Results As compared with MM BMSCs, hUCBDSCs markedly suppressed the proliferation of the KM3 cells. KM3 cells in the KM3/MM-BMSCs co-culture group were arrested in G0/G1 phrase ,while KM3 cells in the KM3/hUCBDSCs co-culture group predominantly in S phrase. The apoptotic rate of KM3 cells in the KM3/ hUCBDSC co-culture group were higher than that in the KM3/MM-BMSCs co-culture group. Conclusion In comparison with MM BMSCs, hUCBDSCs inhibit the proliferation of KM3 cells and promote the apoptosis. Key words: multiple myeloma; human umbilical cord blood-derived stromal cells; bone marrow stromal cells; proliferation; apoptosis骨髓造血微环境在多发性骨髓瘤(multiple myeloma, MM)的发病及瘤细胞的增殖、分化、凋亡中起重要作用，而骨髓基质细胞作为骨髓造血微环境的重要组成成分，通过与骨髓瘤细胞的直接密切接触或释放各种细胞因子，激活细胞内信号转导途径,从而影响瘤细胞的生长、存活[1]。基质细胞是造血细胞赖以生存的造血微环境，在造血调控中发挥重要作用，同时也是细胞治疗和基因治疗中的一种理想靶细胞[2]。基质细胞除广泛分布于骨髓内，