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Cytoplasmic and Nuclear Protein Extraction Materials: Solution Amount Mass of Solid Other 1M HEPES pH 7.9 100ml 23.83g pH! 0.5M HEPES pH 7.6 10ml --- Dilute Above by 2X and pH 1M MgCl2 10ml 0.9522g --- 3M KCl 10ml 2.2368g --- 1M KCl 3ml --- Dilute above by 3X. Glycerol --- --- --- 0.1M CaCl2 10ml 147mg Note mg! 1M MgAc 10ml 2.145g --- 1M Sucrose 10ml 3.423g --- 250mM EDTA 50ml 4.653g pH 8.1, very tedious 100mM DTT 10ml 154.25mg Note mg! Aliquot and freeze at -20°C 100mM PMSF 10ml 174.2g Note mg! Store at -20°C. Dissolve in EtOH. Reagents: Low Salt Buffer-For 10mL200μL of 1M H