翟瑞仁教授热疗联合低量辐射对乳腺癌免疫功能的影响.doc

热疗联合低剂量辐射对乳腺癌患者造血和免疫功能的影响 翟瑞仁，逄朝霞摘要 目的 热疗联合低剂量辐射对乳腺癌免疫功能的影响。方法 随机分为常规化疗、热疗、低剂量辐射、热疗+低剂量辐射联合治疗4组，常规化疗组给予·m-2，口服，d1-14，甲氨喋呤（MTX）40mg·m-2，5-氟尿嘧啶（5-FU）600mg·m-2，iv，d1、8；热疗组于℃静脉低流量灌注·m-2，·m-2，恒温持续0min；低剂量辐射组给予0Co γ射线照射0.01Gy/d5次/周3周；联合治疗组给予热疗+低剂量辐射治疗，方法同上。观察治疗后IL-2）、IL-4、γ干扰素（TNF-γ）、CD4、CD8水平的变化。结果 热疗、低剂量辐射、热疗+低剂量辐射联合治疗组IL-2IL-4、TNF-γ、CD4、CD8水平均高于常规化疗组，热疗组、联合治疗组值均高于低剂量辐射组（P＜0.05），联合治疗组优于热疗组，但差异无统计学意义（P﹥0.05）（P＜0.05）。结论 热疗低剂量辐射。 WANG Li LIU Guangyan. 1Department of Hematology and Oncology, 2Political department, Lao-shan Branch, No 401 Hospital of PLA, Shandong Qingdao 266101, China [Abstract] Objective To study the effect of thermotherapy combined with low-dose irradiation on hematopoietic and immune function of mammary carcinoma. Methods 40 mammary carcinoma patients were randomly divided into conventional chemotherapy group, thermotherapy group, low-dose irradiation group and combination therapy (thermotherapy plus low-dose irradiation) group. Cyclophosphamide 100mg/m2 was administrated orally from 1th to 14 day, Methotrexate 40mg/m2, 5-Fluorouracil 600mg/m2 was administrated intravenously on 1th and 8th day. The same dose Methotrexate and 5-Fluorouracil was primed in low flow and maintain in the constant temperature for 120 minutes in Thermotherapy group. Mice were totally body irradiated by 0.01Gy/d of 60Co-γ ray for 5 days per week for 3 weeks in Low-dose irradiation group. The dose and method of thermotherapy and low-dose irradiation in combined therapy group were same as in thermotherapy group and low-dose irradiation group. Peripheral blood, including white blood cell, red blood cell and platelet, the level of interleukin 2, interleukin 4, γ interferon, CD4+ T,CD8+ T were observed after the above treatments. Results The level of interleukin 2, interleukin 4, γ interferon, CD4+T,CD8+T in thermotherapy group, low-dose irradiation group and combination therapy group were significantly higher than that of the conventional group. The observed index in thermotherapy