G2transcriptioninprokaryotes摘要.ppt

G2 TRANSCRIPTION IN PROKARYOTES promoter 启动子 initiation 起始 elongation 延伸 termination 终止 coding strand 编码链 template strand 模板链 sense strand 正义链 antisense strand 反义链 heloenzyme 全酶 core enzyme 核心酶 transcription bubble 转录泡 Q2：Tuberculosis (TB, 结核病) is an infection caused by Mycobacterium Tuberculosis (结核分枝杆菌)，why Rifampicin（利福平 ）can be used in the treatment of TB? Q2：Tuberculosis (TB, 结核病) is an infection caused by Mycobacterium Tuberculosis (结核分枝杆菌)，why Rifampicin（利福平 ）can be used in the treatment of TB? Nine million new TB cases per year. TB kills 1.5 million people annually. 1 in 3 individuals (worldwide) infected with MT.? transcription translation Gene expression in prokaryote and eukaryote Three phases of transcription (1) Initiation (2) Elongation (3) Termination Coding strand (sense strand) Template strand (antisense strand) The name of DNA strands in transcription (1) Only the template strand is used for the transcription, but the coding strand is not. (2) Both strands can be used as the templates. The transcription direction on different strands is opposite. Asymmetric transcription （不对称转录） 2 Promoters and initiation In E. coli, all genes are transcribed by a single large RNA polymerase with the subunit structure α2ββ’ωσ. Subunit MW Function ? 36512 Determine the DNA to be transcribed ? 150618 Catalyze polymerization ?? 155613 Bind open DNA template ? ω 70263 11000 Recognize the promoter for synthesis initiation unclear RNA-pol of E. Coli ? ?? ? ? ? ? Rifampicin （利福平 ）, a therapeutic drug for tuberculosis （结核病）treatment, can bind specifically to the ? subunit of RNA-pol, and inhibit the RNA synthesis. Promoter: During initiation, RNA polymerase recognizes a specific site on the DNA, upstream from the gene that will be transcribed, called a promoter site. transcribe T T G A C A A A C T G T -35 Pribno