骨髓间充质干细胞分泌基质细胞衍生因子1保末护心肌细胞.docxVIP

31:8080/Jweb_zzgc中国组织工程研究??31:8080/Jweb_zzgc/CN/article/showTenYearVolumnDetail.do?nian?31:8080/Jweb_zzgc/CN/article/showTenYearVolumnDetail.do?nian=2013Vol. 17??31:8080/Jweb_zzgc/CN/volumn/volumn_1153.shtmlIssue (6): 963-968DOI: 10.3969/j.issn.2095-4344.2013.06.003骨髓干细胞骨髓间充质干细胞分泌基质细胞衍生因子1保护心肌细胞Stromal cell-derived factor-1 from bone marrow mesenchymal stem cells protects myocardial cells全文:?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtmlPDF?(531 KB) ?摘要?背景：有研究显示表达CXCR4的干细胞能够沿着基质细胞衍生因子1的浓度梯度迁移到心肌梗死部位再生心肌和血管而改善心脏的功能。目的：探索间充质干细胞通过其分泌的基质细胞衍生因子1对心肌细胞的保护作用。方法：收集培养2 d的间充质干细胞条件培养基。在缺氧条件，利用基质细胞衍生因子1受体CXCR4阻断剂AMD3100或PI3-K/Akt途径阻断剂LY294002预处理H9C2细胞后，利用AnnexinV/PI双标法流式细胞术分析间充质干细胞条件培养基作用下H9C2细胞凋亡的变化；Western blotting分析H9C2细胞磷酸化Akt蛋白的表达；RT-PCR分析间充质干细胞基质细胞衍生因子1的表达。结果与结论：RT-PCR结果显示间充质干细胞表达基质细胞衍生因子1，Western blotting结果显示间充质干细胞条件培养基增加了H9C2细胞磷酸化Akt蛋白的水平。AnnexinV/PI分析发现间充质干细胞条件培养基明显降低了H9C2细胞缺氧复氧后的凋亡，且这种抗凋亡作用能被CXCR4阻断剂AMD3100或PI3-K/Akt途径阻断剂LY294002所阻断。说明间充质干细胞通过其分泌的基质细胞衍生因子1通过激活PI3-K/Akt途径保护H9C2细胞，增加H9C2细胞的幸存能力。关键词?：?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtml干细胞；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtml骨髓干细胞；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtml间充质干细胞；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtmlH9C2细胞；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtml心肌细胞；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtml心肌梗死；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtml基质细胞源衍生因子1；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtml凋亡；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtmlPI3-K/Akt；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtml国家自然科学基金；?31:8080/Jweb_zzgc/CN/abstract/abstract471.shtml干细胞图片文章；?Abstract：BACKGROUND:?Several studies have demonstrated that stem cells expressing CXCR4 can migrate into myocardial infarction region to improve the heart function by regenerating myocardial tissue and vessels under the gradient concentrations of stromal cell-derived factor-1.OBJECTIVE:?To investigate the protective effect of stromal cell-derived factor-1 from bone marrow