HPLC法测定注射用肌苷的含量.docVIP

HPLC法测定注射用肌苷的含量 【摘要】 目的 用高效液相色谱法测定注射用肌苷的含量。方法 采用Kromasil C18柱作流动相，在248nm紫外波长检测。结果 肌苷在～μg/ml范围内有良好的线性关系分别为%、%、%，RSD为%。结论 该法简便、快速、准确，可作为测定注射用肌苷中肌苷的含量。 【关键词】 注射用肌苷；高效液相色谱法；含量测定 【Abstract】 Objective A high-performance liquid chromatography was established for the determination of inosine contents in injection. Methods Impurity in the inosine injection could be separated on Kromasil C18 colnmn using methanol-water as mobile phase and UV detector at 248nm. Results The linear range was ～μg/ recovery in the lower,moderate and higher