MicroRNA-21在卵巢癌中的表达及其与临床病理因素之间的关系.docVIP

MicroRNA-21在卵巢癌中的表达及其与临床病理因素之间的关系 　　 　　[摘要] 目的 检测卵巢癌组织中microRNA-21基因的表达，并探讨其与临床病理因素间的关系。 方法 采用实时定量PCR方法检测64例卵巢癌组织和30例正常卵巢组织中microRNA-21的相对表达量，并分析microRNA-21的表达量在不同临床病理因素间的差异。 结果 在正常卵巢组织、卵巢癌组织中microRNA-21的相对表达量分别为4.23±1.02、7.64±0.87，其差异具有统计学意义（P0.05）。 结论 microRNA-21的表达量在卵巢癌组织中较正常卵巢组织显著增加，其异常高表达可能与卵巢癌的发生、发展有关。 　　[关键词] MicroRNA-21；卵巢癌；qRT-PCR 　　[中图分类号] R737.31[文献标识码] A[文章编号] 1674-4721（2014）05（b）-0050-03 　　 　　Expression of MicroRNA-21 in ovarian cancer and its relationship with clinical pathological factors 　　ZHAO Le1 YANG Guo-dong2 　　1.Department of Clinical Laboratory,the First Hospital Affiliated to Henan University of Science and Technology,Luoyang 471003,China;2.Department of Vascular Surgery,the First Hospital Affiliated to Henan University of Science and Technology,Luoyang 471003,China 　　[Abstract] Objective To detect the expression of microRNA-21 gene in ovarian cancer issue,and to explore its relationship with clinical pathological factors. Methods The relative expression amount of microRNA-21 of ovarian cancer tissue in 64 cases and normal ovarian tissue in 30 cases was detected by quantitative real-time PCR,and the difference of the expression amount of microRNA-21 in different clinical pathological factors. Results The relative expression amount of MicroR-21 in normal ovarian tissue and ovarian cancer tissue was 4.23?.02 and 7.64?.87 respectively,and there was a statistical difference between the two (Pmi＜0.05);the relative expression amount of the ymph node metastasis group and the lymph node metastasis group was 7.24±0.91 and 4.99±1.02 respectively,and there was a statistical difference between the two groups (P＜0.05);the relative expression amount of microRNA-21 of ovarian cancer tissue 0-Ⅱstage and Ⅲ-Ⅳ stage of clinical stage was4.42±0.82, 7.75±1.13 respectively,and there was a statistical difference between the two(P0.05). Conclusion The expression of microRNA-21 is higher in ovarian cancer tissue compared to normal ovarian tissue,the abnom