人正常食管黏膜上皮细胞的纯化分离和传代培养.docVIP

人正常食管黏膜上皮细胞的纯化分离和传代培养 作者：张茹，龚均，王晖，王利，雷娟，冉力伟 【关键词】 细胞培养 Isolation and subculture of human esophageal squamous epithelial cells 【Abstract】 AIM: To investigate the optimal method for the culture of highly purified human normal esophageal epithelial cells and to yield significant cell numbers. METHODS: Cells were isolated from tissues by dispase digestion andtrypsinization and then primarily cultured and subcultured in serumfree keratinocyte medium or fetalbovineserum supplementary medium . The biological characteristics of the ce