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Unit 4, Lesson 18 - Artificial Insemination Part III Page Colorado Agriscience Curriculum Unit 4 –Reproduction and Genetics Lesson 18 Artificial Insemination Part 3 Finding the Target on a Reproductive Tract 1.Lay out trash bags on tables. 2. Lay well drained and thawed reproductive tracts on top of the garbage bags. 3. Obtain a straw from the freezer and load it onto a syringe. Place a paper towel around the end of the syringe and put it in your tucked -in shirt. 4. Put a shoulder-length glove on the left hand and a latex glove on the right hand. 5. Use your left hand to feel the reproductive tract from vagina to cervix. 6. Once you have found the cervix have another student part the vulva, with gloved hand for you to insert your syringe. 7. Insert your syringe at a 35 – 45 degree angle. This may not be as possible with the reproductive tract, but is necessary when performing A.I. on cattle. If you don’t insert the syringe at the right angle in cattle you will end up in the bladder and get wet on. 8. Push the syringe in gently until you feel the cervix. 9. Pinch off the blind pouch with your left hand and use your little finger to guide the rod into the small opening of the cervix. 10. Once in the opening of the cervix, feed the rod through the cervix by moving the cervix around the rod. Do not force the rod by pushing the rod through the cervix. The more damage you cause the less chance of pregnancy. 11. Keep feeding the rod through the cervix and keep your index finger on the anterior end of the cervix. Once you feel the rod hit your index finger you are on target. Make sure not to enter the uterus as you may cause damage. 12. It is here that you will deposit semen. Depositing Semen and Removing the Syringe from the Reproductive Tract. Once at the target, deposit the semen very slowly by pushing the plunger slowly. Once all of the semen is deposited, slowly pull syringe out. Do not pull out too quickly, as the syringe will crea
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