代谢组学的进展教程.ppt

：1.不同物种在相同条件下 存在差异； 2.相同物种在不同储藏 条件下存在差异，储藏 条件越好，储藏时间 越长； 3.相同物种，在理想状态 储藏条件下储藏， 是 一定的。 温度恒定，变化含水量，可以推算 推算出 与含水量的关系： K: 回归的截距 Cw: 斜率 m: 含水量 含水量越低， 越高， 种子保藏时间越长 3.合作的探索：种子激素水平变化 3.合作的探索：种子的脂肪酸 3. 合作的探索 战术合作 技术服务（生理生化指标的检测，各层次观测） 项目合作（投标具体的科学问题，设计解决方案） 战略合作 长期的科研伙伴 种子生物学的研发和保藏管理 3.合作的碳素：基因组学研究 请张旭东博士讲解…… 3.合作的碳素：种质库暨种子保藏中心介绍 感谢关注！ 敬请指教！ 昆明植物所 李唯奇 * * * In order to identify genes associated with fragrant production, microarray was performed with 350 selected unique gene from FC. Based on classification on table 2, these genes covered following putative functions: primary and secondary metabolism and organization, cell rescue, signal transduction, and the ESTs with unknown function. 2 independent RNA extracts. One was hybridized with genes on slide A, another was hybridized with slide B. Before analyzing the gene expression, they examined the reliability of microarray by counting the correlation of expression in A and B RNA probes in A and B were extracted independently from same treatment. Therefore, the expression of a gene should be same if experimental process is reliable. So, they count the comparing the expression ratio between A and B slides. * It is likely associated with fragrance production. * In control, FPP reacted with empty-vector lysates, there was nothing detected. But in the reaction with FC0592-cloned-vector lysates, they did find a type of sesquiterpene, it was germacroen D. At M/Z 204, they got a peak, which is potential Dermacrene, a type of sesquiterpene. No peak in control. The putative Germacere D was further confirmed in figure 6B by comparing mass spec 怎样做的：切片？ * 兰花种子，特性？发育不全，没有营养，萌发中需要从培养基供给。 蓝色，细胞核。红色，油体（分散的状态）。 淀粉也能做。 * 去种皮，未萌发，胚根，细胞壁。 * 细胞壁和油体同时染色 * v = final viability (expressed as %, NEDs or probits) after p days storage.P = storage time (days)m = % moisture content (fresh weight basis)t = temperature (°C)Ki = initial viability of the seed lot at p = 0 d