低磷条件下熊猫豆光合特性及碳水化合物累积变化影响解决方案.doc

低磷条件下熊猫豆光合特性及碳水化合物累积变化影响研究 廉满红1 田宵鸿2 曹翠玲 (西北农林科技大学 1生命科学学院 2资源环境学院 陕西杨凌 712100) 摘要:利用营养液培养方法研究了熊猫豆对低磷胁迫及时间的生理响应。结果表明，磷胁迫下熊猫豆根系最大根长增加46%；但是缺磷时叶片面积变小，且发育延迟。缺磷时熊猫豆幼苗叶片光合速率、气孔导度、叶绿素含量以及蒸腾速率均下降，但是胞间CO2上升；缺磷植株还原性糖、可溶性总糖含量均降低，但是还原糖向根系的分配比例增加；缺磷时熊猫豆根、茎、叶中磷含量均降低；缺磷植株根系活力上升、酸性磷酸酶活性上升。低磷胁迫下熊猫豆干物质累积量显著降低；地上部分干物质累积减少，根系干物质累积增加，因此低磷胁迫导致植物根冠比增大。 关键字:磷胁迫；光合作用；碳水化合物分配；磷含量；酸性磷酸酶活性 Effect of phosphorous stress on photosynthesis characteristics and accumulation of carbohydrate of phaseolus coccineus L seedlings LIAN Man-hong1 TIAN Xiao-hong2 CAO Cui-ling1 (1 College of Life Sciences， College of Resource and Environment，Northwest A F University，YanglingShaanxi 712100，ChinaAbstract:Effect of phosphorus levels and treatment time on some physiological response of phaseolus coccineus L. were studied by hydroponic culture method. The results show that the root length under the no phosphorus application treatment(P2) is about 46% higher than that of the control, ,while the leaf area is decreased when the culture solution is no phosphate. When there is no phosphorus supplied, the root activity and acid phosphatase activity are increased.Under the phosphorus deficiency, both photosynthetic rate and chlorophyll content are decreased, intercellular CO2 is increased; both reducing sugar content and total soluble sugar content are decreased；phosphorus content is decreased in leaf、stem and root；root activity and acid phosphatase are increased. Under the phosphorus deficiency the dry mass is decreased remarkably；shoot dry mass is decreased，root dry mass is increased.These results show that the phosphorus deficiency affects the root top ratio accumulation. Keywords: phosphorous deficiency; photosynthesis; distribution of carbohydrates; phosphorus content;acid phosphatase activity 磷是电子传递、光合磷酸化、CO2固定、卡尔文循环、同化物运输和淀粉合成中的结构组分,对光合作用有重要调节作用[1]，同时磷元素还直接参与作物光合作用的光合磷酸化和碳同化过程。研究表明，当磷供给量较低并成为限制因素时，对植物叶片的扩展产生显著影响，即叶片总叶面积、叶片生长速率和叶片数量显著减少，从而降低了同化面积并严重限制光合作用的总碳固定量[2]。 熊猫豆是我国河西走廊的珍稀名贵彩色豆种适应性较强，具有耐旱、耐低温、耐瘠薄等