MALDI-MS-MiddleTennesseeStateUniversity.pptVIP

Bacterial Identification Rapid bacterial identification is useful in diagnosing disease, monitoring contamination, etc. Important to identify related species Also identify strains in complex matrices Identified by: Biomarkers Cellular protein content MALDI-TOF-MS Bacterial Identification MALDI-TOF-MS uses crude protein extract requiring minimal sample preparation Masses obtained of unknown is compared to experimentally determined signals Ions are specific to genus, species, or strain of bacteria MALDI-TOF-MS can determine mass of proteins of 1-40 kDa [10] Accuracy of ? 0.1% Due to the varia