慢性低灌注脑损伤后失交感神经支配对脑动脉生成-第三军医大学学报.docVIP

慢性脑低灌注后失交感神经支配对脑动脉生成的影响 邹璨，郝磊，田洪，刘磊( 【摘要】 目的 探讨在慢性脑低灌注情况下，失交感神经支配对脑动脉生成的影响。 方法 通过结扎右侧颈总动脉8周建立慢性脑低灌注模型，外科手术法去除同侧颈上交感神经节。将健康的成年雄性SD大鼠随机分为动脉结扎+去神经组(联合组)、动脉结扎组、去神经组和假手术组（每组21只）。术后8周，将每组动物随机分成A、B、C 3个亚组（每组7只）：分别行乳胶灌注、大脑中动脉永久性阻塞以及免疫组化染色。 结果 联合组右侧软脑膜吻合支直径明显小于动脉结扎组，大于去神经组和假手术组［F=22.4，（40.1±5.9）μm，（47.4±6.0）μm，P0.05；（27.8±2.3）μm，P0.05；（33.7±3.6）μm，P0.05］； 联合组右侧Willis动脉环直径明显小于动脉结扎组，大于去神经组和假手术组［F=414.9，（322.0±23.0）μm，（504.3±11.1）μm，P0.05；（223.4±10.5）μm，P0.05；（245.3±18.3）μm，P0.05］；免疫组化结果显示，联合组右侧软脑膜吻合支管壁厚度小于动脉结扎组，大于去神经组和假手术组［F=201.7，（6.9±0.3）μm，（8.0±0.5）μm，P0.05；（2.8±0.4）μm，P0.05；（4.2±0.6）μm，P0.05］；8周后行永久性大脑中动脉阻塞实验，联合组显示大脑中动脉区的脑血流值与基线值的比值小于动脉结扎组，大于去神经组与假手术组［F=36.3，（27.6±4.3）%， （35.7±4.5）%，P0.05；（16.3±3.4）%，P0.05；（20.7±2.4）%，P0.05］；神经行为学评分结果显示联合组评分大于动脉结扎组，小于去神经组和假手术组［F=20.0， 1.7±0.5， 0.7±0.7，P0.05； 3.3±0.5，P0.05； 2.4±0.8，P0.05］；同时TTC染色显示联合组脑梗死体积百分率大于动脉结扎组，小于去神经组和假手术组［F=33.9，（32.0±6.7）%，（18.1±4.2）%，P0.05；（50.4±7.6）%，P0.05；（42.6±6.3）%，P0.05］。结论 脑血管失交感神经支配损害了慢性脑低灌注时的脑动脉生成。 【关键词】慢性脑低灌注；脑动脉生成；交感神经；失交感神经支配 The effect of sympathetic denervation on cerebral arteriogenesis after chronic?cerebral?hypoperfusion Zou Can1, Hao Lei2, Tian Hong2, Liu Lei2. （1Postgraduate?Department?of?the?Third Military Medical University, Chongqing 400038, China; 2Department of neurology, 324th hospital of Chinese PLA, Chongqing 400020, China） 【Abstract】 ObjectiveTo explore the effect of sympathetic denervation on cerebral arteriogenesis in chronic?cerebral?hypoperfusion models of rats and possible mechanism. Methods Chronic?cerebral?hypoperfusion model was established by right common carotid artery ligation for 8 weeks, while denervation was performed by surgical removal of the ipsilateral superior cervical sympathetic ganglion. The healthy adult male SD rats were randomly divided into four groups including arterial ligation combined with denervation group(combined group) (n=21), artery ligation group(n=21), denervation group(n=21) and sham group(n=21). Eight weeks after surgery, the rats in each gr