樹脂相分光光度法测定生物样中微量铁(嘎日迪).docVIP

树脂相分光光度法测定生物样中微量铁 石东辉1 峰 山2 （1.上海市立达职业技术学院护理系，上海，201690；2.上海市医药股份有限公司沪西分公司.上海, 200040） 摘 要 ： 铁（??）与2,2′—联吡啶在pH值5左右的HAc-NH4Ac缓冲溶液中生成有色络阳离子，与强酸性阳离子交换树脂交换吸附，可进行树脂相分光光度法测定痕量Fe(??)。最大吸收波长λmax=522nm，表观摩尔吸光率为9.8×104L?mol-1?cm-1，比水相中直接分光光度法测定Fe(??)的摩尔吸光率提高11倍多。铁（??）的含量在0.2~20mg?L-1范围内符合Beer定律。用此法测定了红豆、黑豆、荞麦和莜麦中的微量铁，相对标准偏差为1.9%~3.5%，回收率在97.8%~103%之间，结果令人满 意。 关 键 词：树脂相光度法 ；2,2′—联吡啶铁；铁 中图分类号： 0657.324 文献标识码：A 文章编号： Resin-Phase Spectrometry Analysis for Trace Iron in Biological Sample SHI Dong-hui1 FENG Shan2 (1:Paramedical School, LIDA Professional College, Shang Hai, 201690 2:HUXI Subsidiary Company, Medicine Co. Ltd. Shang Hai, 200040) Abstract: A new method for determination of trace iron by resin phase spectrometry was studied in this paper. Fe(Ⅱ) and 2,2′- bipyridine can form a colored complex cation in HAc-NH4Ac buffer solution at pH 5.0 and the colored complex cation was adsorpted by strong acidic cation changeable resin, according to this, trace Fe(Ⅱ) can be determined by resin phase spectrometry. The maximum wavelength is at 522nm. The apparent molar absorbance is 9.8×104L?mol-1?cm-1, which is ten times sensitive than that of direct water phase spectrometry. The Beer’s law was obeyed when Fe(Ⅱ) was in the range of 0.2~20mg?L-1. the result was applied to determine the trace iron in small red beans、black soy beans、buckwheat and Avena chinensis with relatively higher sensitivity and precision. The relative standard deviations and recoveries of which are 1.9%~3.5% and 97.8%~103% respectively. Keywords: Resin-Phase Spectrometry; 2,2′—bipyridine; Iron 树脂相（固相）分光光度法报道较多[1~7]。此法的特点是分离、富集和显色等同时进行，所以灵敏度高、选择性好、操作简单、快速。2,2′—联吡啶是在Co、Cu、Mn和Zn存在下，可测定药物制备中Fe[8]的优良试剂，本工作利用铁（??）与2,2′—联吡啶在pH值5左右的HAc~NH4Ac缓冲溶液中生成有色络阳离子，吸附富集于732型强酸性阳离子交换树脂上，有色络合物树脂相分光光度法测定了红豆、黑豆、荞麦和莜麦等生物样中的微量铁，其含量分别为39.87μg?g-1、78.59μg?g-1、45.90μg?g-1、37.34μg?g-1，此结果与原子吸收分光光度（AAS）法 收稿日期： 作者简介：石东辉（1967-），女，讲师，主要从事工作护理及医学，E-mail: 测定结果基本一致 。 1 实验部分1.1 仪器与试剂 722型分光