2.第二章植物发育生物学方法例析.ppt

2. Methods for plant developmental studies 2.1 Materials chosen 2.2 General methods 2.3 Exploring cell lineage 2.4 Genetic mapping 2.5 Gene discover 2.6 Gene expression profiling 基因表达谱分析 Profile of gene expression Number of protein genes 25,498 Number of protein genes 25,498 Profile of gene expression Number of protein genes 25,498 Functional genomics Profile of gene expression Hybrid F1 Parents Total RNA Total RNA Cy5-labeled Cy3-labeled Mix Rice cDNA Microarray Hybridization Schematic representation of the procedures of cDNA microarray analysis Profile of gene expression Drought stress Control Expression profile of 5760 cDNA fragments of unique sequences. The RNA was extracted from the seedlings of ITATA 109 with or without drought stress 数字化表达谱分析 物种名称 基因组大小 测序方式 发表杂志 发表时间 Grapevine 504.6M sanger454 PLoS One 2007 Panda 2.3Gb solexa Nature 2009 Cumcuber 350Mb Solexasanger Nature genetics 2009 Invasive argentine ant 250.8Mb 454solexa PNAS 2010 Turkey 1.1G 454solexa PLoS Biology 2010 Red harvester ant 250-280Mb 454 PNAS 2010 Solenopsis invicta(ant) 352.7Mb 454solexa PNAS 2010 Apple 742.3Mb sanger454 Nature genetics 2010 Theobroma cacao 430Mb sanger454 Nature genetics 2011 Strawberry 240Mb 454solexasolid Nature genetics 2011 Jatropha curcas 400Mb Sanger454 DNA Research 2011 目前已经采用第二代测序技术进行基因组测序的高等动植物列表如下：? 2. Methods for plant developmental studies 2.1 Materials chosen 2.2 General methods 2.3 Exploring cell lineage 2.4 Genetic mapping 2.5 Gene discover 2.6 Gene expression profiling 2.7 Biostatistics and bioinformatics 生物统计和生物信息学 A DH population with 200 lines was constructed from F1 plants of Tapidor DH X Ningyou7 DH Characteristics of the parents of mapping population Mean 平均值 Standard Deviation 标准差 Variance Analysis 变异系数 Biostatistics 烟草PSAG12-IPT转基因植株与野生型植株的比较 6.3 Programmed cell death in different parts of plant 2. Methods for plant developmental studies 2.1 Materials chosen 2.2 General methods 2.3