几种抗肿瘤药物诱导jurkat细胞凋亡的研究.docVIP

几种抗肿瘤药物诱导Jurkat细胞凋亡的研究 【摘要】nbsp; 　　本研究评价几种临床常用的抗肿瘤药物顺铂（CDDP）、羟基喜树碱(HCPT)、高三尖杉酯碱(HHT)和米托蒽醌(MIT)在白血病治疗中的作用，并分析其在诱导Jurkat细胞凋亡的作用，为白血病的临床治疗提供新的理论依据和思路。用Annexin V/PI双参数流式分析方法，检测这几种抗肿瘤药物诱导Jurkat细胞凋亡和死亡的效应。结果表明：MIT和HCPT在加药处理早期4小时 (Plt;0.05)和后期第8小时(Plt;0.05)都有明显的诱导Jurkat细胞凋亡的效应。HHT有明显诱导Jurkat细胞凋亡的效果，但各剂量组之间无显著差异。CDDP在高浓度和长时间作用时才显示较弱的细胞凋亡效应，明显弱于前3者。各种药物作用后均未观察到明显的细胞死亡效应。结论：MIT诱导Jurkat细胞凋亡的作用最显著，Annexin V流式分析方法可作为一种可靠的临床药物筛选的方法。 【关键词】nbsp; 抗癌药物；细胞凋亡；Jurkat细胞系；Annexin V 　　Effect of Several Anti-tumor Drugs onnbsp; Apoptosisnbsp; Induction innbsp; Jurkat Cell Line 　　Abstractnbsp;nbsp; Cisplatin (CDDP)，homoharringtonine(HHT)，mitoxantrone(MIT) and hydroxycamptothecin(HCPT) are highly effective anti-tumor drugs. To evaluate their effectsnbsp; in the therapy of leukemia and establish a valuablenbsp; methodnbsp;nbsp; to estimate anti-tumor drugs，Annexin V/PI double parameter flow cytometry was usednbsp; to detect the effects of these drug inducing apoptosisnbsp; and death in Jurkat cell line.nbsp; The results showed that MIT and HCTP-induced apoptosis effects on Jurkat cellnbsp; line werenbsp; obviousnbsp; at 4 hours in early phasenbsp; after adding drug (Plt;0.05) andnbsp; at 8 hours in late phase after adding drug (Plt;0.05).nbsp; HHT had obvious effect onnbsp; inducingnbsp; apoptosis of Jurkat cells，but no significant difference from lownbsp; to high doses.nbsp; The effect of CDDP on inducing apoptosis of Jurkat cell line was obviously weaker than that of HHT，MIT and HCPT，its weak effect on apoptosis of Jurkat cell line was found only at high concentration of drug for long time.nbsp; Death effects on Jurkat cell line can notnbsp; be observed in every experimental group.nbsp; It is concluded that low dose of MIT can effectivelynbsp; induced apoptosis of Jurkat cell line. Annexin V/PI double parameter flow cytometry can be used as a reliable method for clinical screening anti-tumor drugs. 　　Key wordsnbsp;nbsp;nbsp; anti-tumor drug; apoptosis; Jurka