超临界萃取洛伐他汀(精细化工).docVIP

超临界CO2萃取红曲霉发酵液中的洛伐他汀 王一婕1，徐捷2，周慧3, 曾品涛4 （1.山西中医学院实验中心，山西 太原 030024；2.山西省中西医结合医院 山西 太原 030001；3.山西医科大学科研实验中心，山西 太原 030001；4.宜宾市南溪区科技局，宜宾， 644100） 摘要：作者采用超临界CO2萃取技术提取洛伐他汀，考察了萃取压力、萃取温度、萃取时间及夹带剂用量对洛伐他汀萃取率的影响，并以正交试验优化了超临界CO2萃取洛伐他汀的最佳工艺条件：萃取温度55℃，萃取压力25MPa，萃取时间100min，夹带剂用量为2mL·g-1 ；各工艺参数对萃取率的影响排序为：萃取压力＞萃取温度＞萃取时间＞夹带剂用量。最佳工艺条件下洛伐他汀萃取率可达97.17％。 关键词：超临界CO2萃取；洛伐他汀；正交试验 中图分类号： Study on lovastatin by Supercritical CO2 Extraction WANG Yi-jie1, XU Jie2, ZHOU Hui3, ZENG Pin-tao4 (1, Experimental center, Shanxi University Of Traditional Chinese Medicine, Shanxi 030024,China;2,Shanxi Hospital of Integrated Traditional and Western Medicine, Shanxi 030001,China; 3. Experimental center , Shanxi Medical University, Shanxi 030001,China; 4, The science and technology bureau at Yibin, Sichuan 644100,China ) Abstract：Traditionally，ester was used to extract lovastatin from fungus dreg，which caused environmental pollution，safety problems and solvent residue in diosgenin product．Application of supercritical CO2 extraction might be an effective way to solve these problems, but the extraction conditions and effects on lovastatin product were not yet known．In this paper，it was showed that， 95% ethanol as entrainer and it was twice as much as the raw material, the extraction with supercritical CO2 at 55℃for 100min under 25 MPa got most lovastatin products．According to influence priority ，factors were sequenced as：extraction pressure, extraction temperature, extraction time, and the amount of entrainer. The extraction rate was 97.17％ under the best extraction conditions．Besides，the lovastatin product has high purity with less residual solvent． Key words: Supercritical CO2 extraction；；Orthogonal test TC-SPE-50-0.5-210S型0.5L超临界萃取仪（沈阳天诚超临界萃取有限公司）；Ultrospec 4300TM紫外分光光度计（美国GE Healthcare）；Waters高效液相色谱仪（Waters1525二元泵，Waters2487紫外检测器）；Breeze液相色谱工作站。重庆大新药业股份有限公司上海信然生物技术有限公司HPLC归一化法测定含量为99.8%)；CO2食品级（99.5%，重庆朝阳气体厂）；其他试剂均为市售分析纯。 1.2 提取方法及实验设计 称取100.0g经干燥的菌渣9份，置超临界萃取釜中，加入适量夹带剂，按程序升温加压，进行动态萃取，CO2流量为4.6 L?