大鼠激素性股骨头缺血性坏死进程中GPR48与TLR4信号表达联控.docVIP

大鼠激素性股骨头缺血性坏死进程中GPR48与TLR4信号表达联控 宋会平1，郑小平1，王志强1，李琪佳2 （1河北联合大学附属医院骨科；2河北联合大学实验中心） [摘要] 目的 探讨GPR48和TLR4在大鼠激素性股骨头坏死组织中的表达变化及其相关性，阐明 。 方法 健康SD大鼠只，随机分实验44只肌注地塞米松10 mg／kg，每周1次和对照组只注射等生理盐水代替激素于2、6、8、10周分别取11只只，进行组织学免疫组化RT-PCR检测。 ①HE染色显示，实验组周骨小梁微小断裂，10周骨小梁断裂范围空骨陷窝免疫组织化学染色GPR48实验组2周后各个时间组P0.05），并呈逐渐降低趋势；TLR4实验组2周后各个时间组P0.05），并呈逐渐上升趋势；实验组不同时间点GPR48与TLR4表达呈负相关（r=-0.821，P0.05）；③RT-PCR检测：GPR48 mRNA实验组内各个时间点之间呈逐渐降低趋势，而TLR4表达则呈逐渐上升趋势；实验组不同时间点GPR48与TLR4表达呈负相关（r=-0.791，P0.05）。 结论 大鼠激素性股骨头坏死发展进程中GPR48和TLR4的表达呈负相关性，共同介导坏死过程中的炎症免疫反应。 [关键词] 激素；股骨头坏死C2011401025）；河北省科技厅资助项目） [通信作者] 王志强， E-mail: wzhq@163.com The co-expression regulation of GPR48 and TLR4 in the progression of steroid-induced avascular necrosis of the femoral head in rats Song Huiping1, Zheng Xiaoping1, Wang Zhiqiang1*, Li Qijia2 （1 Orthopeadic Department of the Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province; 2 Experimental Center of Hebei United University, Tangshan 063000, Hebei Province, China） [Abstract] Objective To analyze the GPR48 expression changes and in steroid-induced avascular necrosis of the femoral head. Methods 68 SD rats were divided into groups randomly, with 44 rats in experimental group (injected dexamethasone sodium phosphate 10mg/kg weekly), and 24 in contral group (injected with an equal volume of normal saline instead of hormone). At 2, 6, 8, 10w, 11 rats in experimental group and 6 rats in contral group were sacrificed and followed by analyzed by histology, immunohistochemistry and RT-PCR detection. Results (1) Histological and quantatitive analyses: in HE stain, experimental group at 2w showed occasionally small micro-fractures, at 10w displayed a wide range of trabecular bone fracture. For quantitative anlysis of Masson trichrome staining, the per unit area of Collagen content in experimental group was significantly higher (P0.05) at 6w, 8w, 10w than that in control group. (3) GPR48 immunohistochemical stain: compared with con