脆弱类杆菌荧光定量pcr法检测.docVIP

脆弱类杆菌荧光定量PCR法检测 【关键词】 脆弱类杆菌；荧光定量PCR；SYBR,Green,I 　　　摘要： 目的 探讨荧光定量PCR技术检测脆弱类杆菌。方法 用特异性引物和荧光染料实时PCR扩增并检测产物。结果 脆弱类杆菌标准株(ATCC 25285)和4株分离株均有特异扩增曲线，灵敏度达 102个菌；而大肠埃希菌、保加利亚乳杆菌、嗜热链球菌无特异扩增曲线。结论 荧光染料实时PCR技术可特异并定量检测脆弱类杆菌 　　关键词： 脆弱类杆菌；荧光定量PCR；SYBR Green I 　　Detection bacteria fragile by SYBR Green real-time PCR 　　Abstract： Objective To identify the bacteria fragiles(B.fragiles)by 16S target rRNA realtime PCR.Methods A pair of specific primers was designed.The PCR products were detected by fluorescent quantificative realtime PCR.Results The B.fragilis ATCC 252