鼠妇水煎物镇痛作用的实验研究.docVIP

鼠妇水煎物镇痛作用的实验研究 【摘要】 目的观察鼠妇水煎物的镇痛作用。方法通过小鼠扭体法致痛、热板法致痛、大鼠甩尾法热刺激致痛实验对鼠妇水煎物进行镇痛活性研究。结果鼠妇水煎物（蛋白含量）90，180，360 mg·kg-1灌胃（ig）对醋酸引起的小鼠扭体反应有明显的抑制作用，抑制率为32.00%～58.15%；灌胃给药后30 min和45 min明显提高小鼠热板痛阈（P＜0.01，P＜0.05），镇痛效果随给药量的增加呈剂量依赖关系。结论 鼠妇水煎物对多种疼痛模型均具有明显的抑制作用并呈剂量依赖关系。 【关键词】 鼠妇 镇痛 水煎物 　　Research on the Analgesic Effects of Water Decoction of Armadillidiunm vulgare in Mice 　　Abstract：ObjectiveTo study the analgesic effects of water decoction of Armadillidium vulgare in mice.MethodsThe analgesic effects of water decoction from Armadillidium vulgare in mice were studied in mice and rats using writhing reaction ,hot plate nociceptive test and tail flick test .ResultsThe analgesic experiment showed that protein of water decoction from Armadillidium vulgre at the dose of 90,180,360 mg·kg-1 had significant inhibition to writhing response (P＜0.01,P＜0.05)induced by acetic-acid.The inhibitory rate was from 32.00% to 58.15%.The pain threshold in mice with the hot-plate procedure increased obviously after being taken water decoction from Armadillidium vulgare for 30 min and 45 min(P＜0.01,P＜0.05).The three groups with different doasges could obviously increase the pain threshold in imce.The pain threshold in rats procedure increased obviously after being taken water decoction from Armadillidium vulgare for 60 min and 90 min in the tail flick test (P＜0.01,P＜0.05).The three groups with different dosages could obviously increase the pain threshold.ConclusionThe water decoction from Armadillidium vulgare has significant analgesic effect on animal models and shows obviously doseeffect ralationship. 　　Key words：Armadillidiam vulgare; Antalgesia; Water decoction 鼠妇为鼠妇科动物平甲虫Armadillidiam vulgave的干燥全体［1］，分布在广东、河北、山东、江苏、浙江等地。主要含多糖、蛋白质、蚁酸，具有解毒止痛、破血利水作用。国内曾有报道［1］，将鼠妇煎煮用于肝癌晚期的止痛，作用持久性优于杜冷丁，民间有用于治疗手术后疼痛。有文献报道［2］，鼠妇中蛋白起镇痛作用。为此，本文以鼠妇水煎物的蛋白含量为指标，采用两种动物三种镇痛模型对鼠妇水煎物镇痛作用进行研究，为进一步开发鼠妇及临床用药提供实验理论依据。 　　1 材料与方法 　　1.1 药物鼠妇干体，购于长春中医学院，并由长春中医学院鉴定。焙干鼠妇，经粉碎后用水煎煮3～4 h，过滤，8