衰老大鼠细胞免疫功能的变化及中药天年饮的延缓衰老作用.docVIP

衰老大鼠细胞免疫功能的变化及中药天年饮的延缓衰老作用 【摘要】 目的观察D-半乳糖衰老大鼠细胞免疫功能的变化及中药天年饮的延缓衰老作用。方法40只SD大鼠随机分为四组，正常组、衰老模型组、天年饮用药组及阴性对照组，每组均为10只。D-半乳糖连续腹腔注射制作亚急性衰老的大鼠模型。采用ELISA方法检测各组大鼠血清IL-2及IL-6的含量，同时检测各组大鼠的胸腺指数和巨噬细胞的吞噬功能。结果衰老大鼠血清IL-2的含量、胸腺指数、巨噬细胞的吞噬功能与正常大鼠相比均明显下降（P＜0.05，P＜0.01），衰老大鼠血清IL-6的含量与正常大鼠相比明显升高（P＜0.01）。天年饮可显著提高衰老大鼠血清IL-2含量、胸腺指数以及巨噬细胞的吞噬功能（用药组大鼠与衰老模型组大鼠比较：P＜0.01，P＜0.05）；天年饮可明显降低衰老大鼠血清IL-6的含量（用药组大鼠与衰老模型组大鼠比较：P＜0.01）。结论天年饮可提高衰老大鼠的细胞免疫功能，具有一定延缓衰老的作用。 【关键词】 天年饮 衰老 细胞免疫 　　Study on the Changes of Cellular Immune Function of Aging Rats and the Anti-aging Effects of Traditional Chinese Medicine Tiannianyin 　　Abstract:ObjectiveTo study on the changes of cellular immune function of aging rats and the anti-aging effects of traditional Chinese medicine Tiannianyin.Methods40 SD rats were divided into 4 groups at random: normal group, aging model group, Tiannianyin group and negative control group, each group had 10 rats. The sub-acute aging model rats were made by injecting D-gal into abdominal cavity continually. ELISA was used to detect the IL-2 and IL-6 content in serum, the macrophagic phagocytosis and thymus index were also observed. ResultsCompared with normal rats, the IL-2 content in serum, the thymus index and the macrophagic phagocytosis of aging rats decreased obviously (P＜0.05，P＜0.01); the IL-6 content in serum increased obviously (P＜0.01). Tiannianyin not only significantly increased the IL-2 content, the macrophagic phagocytosis and the thymus index of aging rats (Tiannianyin group compared with aging group: P＜0.01, P＜0.05); but also significantly decreased the IL-6 content of aging rats (Tiannianyin group compared with aging group: P＜0.01).ConclusionsTianninyin can strengthen the cellular immune function of aging rats, so it has fairly anti-aging effects. 　　Key words:Tiannianyin; Aging; Cellular immunity 研究表明随着年龄的增长，机体的免疫功能发生紊乱并逐步衰退，由于机体免疫功能的下降，从而导致自身免疫疾病以及癌症发生的增多［1］。中药天年饮由刘河间《宣明论方》何首乌丸加味而成，由何首乌、肉苁蓉、怀牛膝等组成，具有补肝肾、益精血、延年益寿之功效。我们的研究已经发现，天年