钛铌锆β钛合金低弹性模量内固定材料的生物相容性研究.docVIP

钛铌锆β钛合金低弹性模量内固定材料的生物相容性研究 【摘要】 目的］通过体内实验的方法对新型β型钛合金（TiNbZr）骨折内固定材料的生物相容性进行评价。［方法］（1）改良的鼠气囊模型（the air pouch model）：健康成年SD大鼠24只，皮下充气造模后将钛铌铣β型钛合金（TiNbZr）颗粒混悬液注入气囊，收集囊内液体采用ELISA方法测定IL6和TNFα，用囊壁组织学切片进行炎症细胞反应分级和囊壁厚度测量，并与传统的钛铅钒合金（Ti6Al4V）进行比较。（2）接骨板植入模型：将TiNbZr做成接骨板固定在兔的胫骨上，于手术后4、8、12、24、36周分别观察接骨板周围的纤维膜形成情况和TiNbZr骨界面骨结合情况，并与不锈钢接骨板比较。［结果］（1）Ti6Al4V、TiNbZr两种颗粒注入气囊48 h后都能引起TNFα分泌量显著升高（Plt;005），两组比较，Ti6Al4V组明显高于TiNbZr组（Plt;005）。两种材料均不能引起IL6分泌的显著增加（Pgt;005）。TiNbZr组气囊囊壁厚度明显小于Ti6Al4V组（Plt;0001）。（2）TiNbZr接骨板周围软组织反应与不锈钢相近。植入后4周，新生骨痂开始覆盖TiNbZr接骨板的两侧，8周时骨痂甚至部分覆盖接骨板和螺钉的表面形成“骨盖板”现象。12周时，骨与TiNbZr接骨板的界面为直接接触，中间无软组织间隔。螺钉与骨也能形成较为牢固的钉骨直接接触。［结论］低弹性模量TiNbZr β钛合金具有优良的体内组织相容性，是一种有前途的骨折内固定材料。 【关键词】 内固定　钛铌锆　生物相容性材料　体内研究 Abstract［Objective］To develop new beta titanium alloys made of titanium, niobium and zirconium (TiNbZr) which have higher strength, lower moduli of elasticity Its biocompatibility in vivo is evaluated in this paper ［Method］(1) 24 SD rats were used in the modified air pouch model experiments Air was injected subcutaneously on the back of rats to form the pouches and the TiNbZr particles were introduced into them The liquid in the pouches was harvested 48 hours later for IL6 and TNFαELISA procedure and the capsules for histology examination All the results were compared with Ti6Al4V (2) TiNbZr bone plates were fixed onto tibias of rabbits The capsules around the plates and the bone under them were observed at 4, 8, 12, 24 and 36 weeks respectively TiNbZrbone interfaces were emphasized in this procedure This time the control material was stainless steel ［Result］(1)TiNbZr and Ti6Al4V particles made the air pouches secret more TNFα than that of the control (Plt;005) Ti6Al4V particles made the effect more prominent than TiNbZr particles did (Plt;005) This effect was not seen in IL6 menstruations experiments The thickness of the air pouches was different in two