调肝脾方对佐剂性关节炎大鼠红细胞免疫功能的影响.docVIP

调肝脾方对佐剂性关节炎大鼠红细胞免疫功能的影响 【摘要】 目的探讨调肝脾方对佐剂性关节炎大鼠红细胞免疫功能的影响。方法采用健脾和肝、除痹止痛为原则组方，以补肝肾方、火把花根为阳性对照组，建立AA大鼠模型，随机分为调肝脾方组、补肝肾方组、芬必得组、火把花根片组、正常对照组、模型组6组，观察各组对AA大鼠足肿胀度及红细胞免疫功能的影响。结果调肝脾方能明显降低AA大鼠原发与继发性关节肿胀度，表现出较好的抗炎作用，并能显著增强红细胞的免疫功能。结论调肝脾方具有明显的抗炎作用，其作用机理与增强红细胞免疫功能有关。 【关键词】 调肝脾方药；佐剂性关节炎；RBC-C3b受体花环率； RBC-IC花环率 　　The Influence of the Means of Attempering the Liver and the Spleen on AA Rats Red Blood Cell Immunity Function 　　　Abstract:ObjectiveTo study the influence of the means of attempering the liver and the spleen on red blood cell immune function in adjucant arthritis (AA) rats. MethodsThe arthritis model was induced by intracutaneously injection of complete Freuds adjurant in the right hind foot pad of rat. Th rats are randomly divided into 6 groups: the means of attempering the liver and the spleen group, the means of complementing the liver and the kidney group, Fenbid group, Huobahuagen pian group, normal control group, and model group and the foot swelling degree and the red blood cell immunity function of AA rats. ResultsThe means of attempering the liver and the spleen could obviously reduce the foot swelling degree, and of AA rats observed showed fairy anti-inflammntory effect. The red blood cell immune function could notably be strengthened. ConclusionThe means of attempering the liver and the spleen has obvious anti-inflammation function, and the mechanism of action is relevant to strengthen the red blood cell immune function. 　　Key words:The means of attempering the liver and the spleen; Adjuvant arthritis; RBCC3b reception body garlend rate; RBCIC garland rate 类风湿关节炎(Rheumatoid arthritis, RA)属于自身免疫性疾病，是一种以周围关节对称性炎症为主、伴反复发作的慢性全身性疾病，属于中医痹证范畴。历代医家对治疗痹证积累了丰富的经验，并根据痹症的病因病机不同，进行不同的组方配伍，如疏风祛湿，化痰祛淤，助阳散寒，养阴清热，舒筋通络，强筋壮骨等，形成了诸多行之有效的治疗方法。笔者经大量临床文献研究［1，2］发现，“肝脾失调”有可能是RA发病的重要基础，且调肝脾方药对RA治疗亦有良好的效果。为进一步探讨中药调和肝脾配伍抗炎增效的作用机制，笔者采用健脾和肝、除痹止痛为原则组方，并以补肝肾方和火把花根作为阳性对照组,探讨调肝脾方的抗炎作用，以及对红细胞免疫功能的影响，为临床治疗RA提供药理学依据。 　　1 材料与方法 　　1.1 动物、分组及造模、给药雄性