去势大鼠骨质疏松症基因表达谱特征初步分析高峰1，莫新民1.docVIP

去势大鼠骨质疏松症基因表达谱特征初步分析 高峰1 ， 莫新民1△，刘仕杰1，张小君1， 李劲平2 （1.湖南中医药大学，湖南 长沙 410007；2.中南大学药学院，湖南 长沙 410013） 摘要: 目的　探讨去势大鼠骨质疏松症与正常大鼠基因表达谱的差异,初步分析去势大鼠骨质疏松症基因表达谱的特征,探讨去势大鼠骨质疏松症的病理生理机制。方法 应用基因芯片技术检测去势大鼠骨质疏松症与正常大鼠相关基因的表达,通过Gene Ontology（GO）数据库对差异表达基因进行基因信息学分析,运用Agilent GeneSpring GX 11软件对差异基因进行GO分析。结果　去势大鼠骨质疏松症共有118条差异表达的基因,其中有48条基因上调,70条基因下调，主要涉及细胞质、发育、代谢调节、结合等方面。结论　去势大鼠骨质疏松症差异表达基因主要涉及细胞质、外周神经系统发育、内皮发育、调节细胞酮体代谢、调节脂肪酸代谢过程、正向调节脂类代谢、正向调节脂肪酸、内质网、染色质重塑、胶原蛋白结合、血-神经屏障的建立、铜离子结合、内皮细胞发育分化、主要组织相容性复合体蛋白复合物等。 关键词: 去势大鼠骨质疏松症;基因表达谱 Preliminary analysis of expression profiles of gene in osteoporosis in ovariectomized rats by cDNA microarry. 　GAO Feng , MO Xinming， LIU Shijie , ZHANG Xiaojun，LI Jinping.（Hunan University of Traditional Chinese Medicine , ChangSha 410007,China; Medicine College,Zhongnan Univesty,Changsha 410013） Abstract: Objective To investigate the osteoporosis based on rat genome chip.Metods In this study ,gene chips of RatRef-12-were used to investigate the gene expression profile in osteoporosis. Results 118 significant differentially expressed genes related to were detected ,among them 48 genes were up regulated,70 down regulated .They related cytoplasm, development ,binding ,metabolic regulation etc.Conclusion Related genes of osteoporosis in ovariectomized rats is closely correlated with cytoplasm,peripheral nervous system development,regulation of fatty acid metabolic process ,cellular ketone metabolic,endothelium development ,cell differentiation,copper ion binding,collagen binding,MCH protein complex, chromatin remodeling,MCH classII protein complex, endoplasmic reticulum, establishment of blood-nerve barrie, positive reglative of fatty acid, positive regulation of lipid metabolic ect. Key words : Osteoporosis ; Gene　expression profiles 骨质疏松症(osteoporosis ,OP) 是一种全身性的骨代谢疾病。原发性骨质疏松症是以骨量减少,骨小梁变细、断裂、数量减少,骨皮质多孔、变薄为特征,并由此导致骨的脆性增高及骨折危险性增加的一种全身性骨病。随着老龄化社会的到来将有更多的骨质疏松症患者，为家庭及社会带来很大负担。骨质疏松症的发生、发展是一个多基因、多步骤、多阶段的复杂过程, 其演化过程涉及大量的基因突变、缺失、扩增及失活等异常表达信息, 因此