化学工艺毕业设计.doc

化学工艺毕业论文 题目: 环境激素辛基酚的电化学检测 专 业 :化学工程与工艺 目 录 摘要 1 1 前言 3 1.1 环境内分泌干扰物 3 1.2 碳糊电极的特点及应用 4 1.3 循环伏安法的特点及应用 4 1.4 生物样品分析 5 2 实验部分 5 2.1 实验仪器及试剂 5 2.2 碳糊电极的制备 5 2.3 实验方法 6 3 结果与讨论 6 3.1 底液的选择 6 3.2 底液pH的影响 7 3.3 富集时间的影响 8 3.4 扫描速度的影响 8 3.5 线性范围、校正曲线 9 3.6 电极的重现性、稳定性 10 3.7 干扰实验 10 3.8 回收率及样品的测定 11 4 结论 11 参考文献 12 致谢 13 附录 13 环境激素辛基酚的电化学检测 摘要：本文研究了辛基酚在碳糊电极上的电化学性质，在-0.21.2V的电位范围内进行了循环伏安扫描，辛基酚在0.7V处有一明显的氧化峰，根据该氧化峰的电流与浓度的关系建立了循环伏安法测定辛基酚的电化学分析方法。对辛基酚检测方法的条件进行了优化。结果表明：测定的最佳底液是pH=5的磷酸盐缓冲溶PBS，扫描速率为100mV/s。辛基酚浓度与氧化峰电流在7.0×10-7~3.1×10-5mol/L范围内呈良好的线性关系，线性方程为：ipa (A)= 2.0×10-6+0.4598 c mol/L,线性相关系数r= 0.9966，该方法的检测限为4.0×10-7 mol/L。用标准加入法对辛基酚样品的回收率进行测定，回收率范围在96.5%～105.4% 之间，平均回收率为99.7%。将该方法用于鲫鱼体内辛基酚含量的测定取较好的结果。该方法具有电极制作简单，操作简便测定结果准确等优点。 关键词：lectrochemical Detection of Environmental Hormone Octyl Phenol Abstract: This paper had investigated the electrochemical properties of the octyl phenol in carbon paste electrodes, cyclic voltammetric scanning was proceeded in -0.2~1.2V potential range, meanwhile, octyl phenol was an obvious oxidation peak at 0.7V place, according to the relationship between the oxidation peak current and concentration, a method of electrochemical analysis called cyclic voltammetry was established to detect octyl phenol. The conditions of detection method for octyl phenol were optimized. The results showed that the optimal determination of the bottom fluid was the phosphate buffer solution (pH=5.0) and scanning rate was 100mV/s. In the range of 7.0×10-7~3.1×10-5mol/L, concentration and oxidation peak currents of ocytl phenol presented a good linear relationship, the linear equation was: ipa (A)= 2.0×10-6+0.4598c (mol/L), the linear correlation coefficient was r=0.9966 and this detection limit of this method was 4.0×10-7mol/L. Standard addition method was used to the detection of recovery of octyl phenol samples, the recovery range was 96.5% ~ 105.4%, and the average recovery was 99.7%. The method applied to detect the content