Mannose修饰超顺磁纳米颗粒的制备及体外试验-第三军医大学学报.doc

甘露糖修饰SPION的制备及体外特性分析贺娟，贾延辉，邓均，李艳，方立超，摘要 目的 探讨以甘露糖（Mannose）修饰超顺磁纳米颗粒superparamagnetic iron oxide nanoparticles，SPION）的制备方法并检测其理学性质体外试验探讨该体系能否被网状内皮系统吞噬从而通过巨噬细胞向肿瘤组织侵润达到对早期癌症显影的目的。方法 采用共沉淀法制备Fe3O4纳米颗粒然后加入甘露糖溶液，在通氮条件下90 ℃剧烈搅拌2，得到甘露糖包被的。采用透射电镜测量其大小和分布，，用体外白细胞吞噬试验检测其能否被吞噬，用细胞毒性试验检测样品的安全性。结果 所得样品为球形或类球形，分散性好，核心为Fe3O4晶体，核心粒径为nm，包被甘露糖后的整体颗粒直径不超过20 nm红外吸收光谱测定结果表明甘露糖Fe3O4的表面体外白细胞吞噬试验表明白细胞对甘露糖SPION的吞噬率的较高细胞毒性实验表明其对细胞的IC50为100.44 μg/ml。结论制备的Mannose-SPION具有粒径小，分散性好，超顺磁性等优点，被吞噬细胞吞噬，细胞毒性较低。 关键词共沉淀法；超顺磁性氧化铁；甘露糖（200）通作者郑峻松，电话：（023E-mail: @ Preparation and in vitro test of Mannose-modified superparamagnetic iron oxide nanoparticles HE Juan1, JIA yan-hui2, DENG Jun1, LI Yang1, FANG Li-chang1,ZHENG Jun-song1 (1Department of Clinical Laboratory Sciences, College of Medical Laboratory, Tird Military Medical University, chongqing 400038, China, 2 75569Forces95 Units, Haikou, 571146, China) 【Abstract】 Objective To explore how to prepare Mannose-modified superparamagnetic iron oxide nanoparticles (SPION) and test the physical properties and in vitro test. Investigate whether the system was fully engulfed by the reticuloendothelial system, thereby achieved the purpose of early cancer imaging by macrophages to tumor invasion. Methods To preparation iron oxide nanoparticles by co-precipitation method. Then Mannose was added to the solution and prepared the Mannose-SPION under high-speed mechanical stirring at 90℃ by 2 hours. The size and distribution were measured by transmission electron microscope (TEM); Modifying effect of mannose were measured by Fourier transform infrared spectroscopy (FTIS); The iron content of samples measured by atomic absorption spectrophotometer (AAS); Whether the granule to be fully engulfed were measured by phagocytic white blood cells in vitro test; The safe of the nanoparticles were measured by cell toxicity test. Results The Mannose-SPION was globular or spherical, and good dispersion. The cores were Fe3O4 and the average core diameter was 12.90?.29e