ImageJ分析粒径尺寸及分布.docxVIP

Morphological study: focal contacts formationFor quantification of vinculin positive contacts areas, we used the freewareimage analysis ImageJ (NIH, /ij/). We opened the raw image,converted it to an 8-bit file, and used the unsharp mask feature (settings 1:0.2)before removing the image background (rolling ball radius 10). After smoothing, theresulting image, which appears similar to the original photomicrograph but withminimal background, was then converted to a binary image by setting a threshold.Threshold values were determined empirically by selecting a setting, which gave the most accurate