亮菌多糖ATPS1a的抗衰老作用研究.doc

亮菌多糖ATPS-1a的抗衰老作用研究 马金宝，沈业寿*，赵根海，卫自 （安徽大学生命科学学院、生化微生物研究所，安徽省中药研究与开发重点实验室 中国 合肥 230039） 摘要：目的：研究亮菌多糖ATPS-1a的抗衰老作用。方法：选用D-半乳糖致衰老模型小鼠，称小鼠体重,测定脾脏、胸腺指数,取血清、肝、脑组织测定SOD、MDA、GSH-Px、NO。结果：亮菌多糖ATPS-1a能明显增加小鼠体重，抑制胸腺指数上升、脾脏指数下降，还能显著提高SOD及、GSH-Px活性并降低MDA、NO含量。结论：亮菌多糖ATPS-1a可通过调节机体免疫功能、清除自由基而发挥其延缓衰老的作用。 关键词：亮菌；多糖；抗衰老；D-半乳糖；小鼠 Study on the Effect of Anti-aging of Polysaccharide ATPS-1a from Armillariella tabescens MA Jin-bao,SHEN Ye-shou*，ZHAO Gen-hai Wei Zi （Institute of Biochemistry and Microbiology，Schooi of Life Science，Anhui University；Anhui Key Laboratory of Research and Development of Chinese Medicine，Hefei 230039，China） ABSTRACT：AIM：To study the anti-aging effects of polysaccharide ATPS-1a from Armillariella tabescens on mouse.METHODS：Regarding D-galactose induced aging mice as research objects，the body weight，thymus and spleen index， Superoxide Dismutase (SOD) and Glutathione peroxidase(GSH-Px) activities，Maleic Dialdehyde (MDA) and nitrogen monoxide (NO) levels in serum，liver and brain were measured.RESULTS：Polysaccharide ATPS-1a from Armillariella tabescens could significantly increase the body weight，markedly resist the rising of thymus index and the dropping of spleen index of senile model mice.It could increase SOD and GSH-Px activities obviously，decrease MDA and NO levels dramatically on senile model mice. CONCLUSION：Polysaccharide ATPS-1a from Armillariella tabescens has anti-aging effects by immune-regulatory and eliminating free radicals. KEY WORDS：Armillariella tabescens；polysaccharide；anti-aging；D-galactose；mouse 亮菌（Armillariella tabescens）属真菌界、担子菌门、层菌纲、伞菌目、白蘑科、小蜜环菌 (假蜜环菌)属[1]。它是我国最初发现并拥有自主知识产权的一种真菌。1969年江苏省丹徒县群众发现用当地发光的柳树根水煎剂能治疗胆囊炎，亮菌成为20世纪70年代新发现的药用真菌，子实体可食用，是一种药食兼用的名贵食用菌。中医认为，该菌性寒、味甘、有强筋壮骨、明目、利肺、益肠胃等保健功效，在我国民间流传作为治疗急慢性肝炎及胆道疾患的中草药[2]。目前，国内外对亮菌多糖的研究主要集中在治疗肝炎、防辐射、升白、抑瘤和调节免疫的药理作用上[3-6]，而对亮菌多糖的抗衰老作用还未见报道，本研究拟对亮菌多糖ATPS-1a的抗衰老作用及其机制进行探讨，旨在寻求有效的抗衰老剂及自由基清除剂，为延缓人体衰老及防治与自由基有关的疾病提供理论依据。 1 材料与方法 1.1 材料 1.1.1 样品与试剂 ATPS-1a(多糖含量为88.76％)由安徽大学糖复合物与糖工程实验室提 供。D-半乳糖，由上海试剂二厂生产。