吸入一氧化氮对大鼠无心跳供体肺缺血再灌注损伤的影响.docVIP

吸入一氧化氮对大鼠无心跳供体肺缺血再灌注损伤的影响 作者：廖东山, 李增棋, 余毅 作者单位：福建医科大学附属第一医院心外科,福州 350005 【摘要】 目的 探讨一氧化氮(NO)对大鼠无心跳供体(NHBD)肺缺血再灌注损伤(IRI)的影响。 方法 大鼠随机分为Ⅰ组:有心跳供体(HBD)组;Ⅱ组:NHBD-吸氧组;Ⅲ组:NHBD-吸氧＋NO组。Ⅰ组在处死的同时灌注4 ℃低钾右旋糖苷液(LPD),Ⅱ组、Ⅲ组处死后维持辅助呼吸,放置室温中30 min再灌注LPD液。供肺置于4 ℃ LPD 液中4 h 后行原位左肺移植术。术后维持辅助呼吸1 h。Ⅲ组通气吸氧的同时吸入浓度为40×10-6的NO。 结果 与Ⅱ组比较,Ⅲ组肺顺应性、动脉血氧分压高,肺组织丙二醛含量低(P0.05)。 结论 吸入低浓度NO可减轻大鼠NHBD肺缺血再灌注损伤。 【关键词】 一氧化氮; 组织供者; 肺移植; 缺血; 再灌注损伤; 疾病模型,动物 　　Inhaled Nitric Oxide for the Ischemia-Reperfusion Injury in Rat Lungs from Non-Heart-Beating Donors 　　LIAO Dongshan, LI Zengqi, YU Yi 　　Department of Cardiovascular Surgery, The Affiliated First Hospital, Fujian Medical University, Fuzhou 350005, China ABSTRACT: Objective To evaluate the effect of inhaled nitric oxide(NO) on the ischemia-reperfusion injury(IRI) in rat lungs from non-heart-beating donors(NHBD). Methods Sixty Sprague-Dawley rats were randomly divided into 3 groups: groupⅠ, heart-beating donor; groupⅡ, NHBD with 30 minutes of warm ischemia time(WIT) and oxygen inhaled; group Ⅲ, NHBD with 30 minutes of WIT and oxygen and NO inhaled. The donor lungs of groupⅠ were flushed with low potassium dextran(LPD) solution at 4 ℃ after asystolia while the lungs of groupⅡ and group Ⅲ remained ventilated at the room temperature for 30 minutes after asystolia and then were flushed with LPD solution. All of the donor lungs remained inflated when they were stored in LPD solution at 4 ℃ for 4 hours. The recipient rats underwent orthotopic left lung transplantation. After transplantation 1 hours reperfusion and ventilation was manipulated. The donor lungs of group Ⅲ inhaled additional NO with the concentration of 40×10-6 at the time of ventilation. Results Compared with the group Ⅱ, the pulmonary compliance and the oxygen partial pressure of the arterial blood are higher, the MDA in the lung lower(P0.05). Conclusion The low-dosage of NO is an effective treatment to attenuate the acute injury resulted from th