刺五加注射液中3个主要活性成分与相应单体在大鼠体内的药.docVIP

刺五加注射液中3个主要活性成分与相应单体在大鼠体内的药动学比较 范惠霞1,2,3，邓志鹏1,3， 仲浩1,3，徐晓婷1,2,3，姚庆强1,3( (1 山东省医学科学院药物研究所, 山东 济南250062; 2 济南大学 山东省医学科学院医学与生命科学学院，山东 济南 250022; 3 山东省罕少见病重点实验室，山东 济南250062) 摘要：研究刺五加注射液中3个活性成分—紫丁香苷、刺五加苷E及异嗪皮啶在大鼠体内的药动学行为，并与相应单体分别经静脉注射给药后大鼠体内药动学行为比较。将24只雄性大鼠随机分为4组，分别经静脉注射给予紫丁香苷、刺五加苷E、异嗪皮啶及相当剂量的刺五加注射液。采用液相色谱-串联四级杆质谱(liquid chromatography tandem mass spectrometry, LC-MS/MS)法测定不同时间点血浆中紫丁香苷、刺五加苷E及异嗪皮啶的浓度DAS 2.0 数据处理软件SPSS 17.0 统计软件药动学参数计算比较。静脉给药后，3种单体成分与刺五加注射液药动学参数-时曲线下面积 (AUC)清除率CL)、消除半衰期 (t1/2)显著性差异 (P ( 0.05)。刺五加注射液中紫丁香苷AUC 较明显增大，CL较组明显减小刺五加苷EAUC 较明显CL较组明显注射液异嗪皮啶较组t1/2。刺五加注射液紫丁香苷、刺五加苷E及异嗪皮啶大鼠体内存在差异。刺五加注射液中的成分pharmacokinetics of syringin, eleutheroside E and isofraxidin in rat plasma after intravenous administration of each monomer and Acanthopanax injection FAN Hui-xia1,2,3, DENG Zhi-peng1,3, ZHONG Hao1,3, XU Xiao-ting1,2,3, YAO Qing-qiang1,3( (1 Institute of Materia Medica, Shandong Academy of Medical Sciences, Jinan 250062 China ; 2 School of Medinice and Life Sciences, University of Jinan and Shandong Academy of Medical Science, Jinan 250022 China ; 3 Key Laboratory of Rare and Uncommon Diseases of Shandong Province, Jinan 250062 China ) [Abstract] To compare the pharmacokinetics of syringin, eleutheroside E and isofraxidin after intravenous administration of each monomer and Acanthopanax injection. Twenty-four Sprague-Dawley rats were randomly divided into four groups and intravenously administrated with syringin, eleutheroside E, isofraxidin, and Acanthopanax injection, respectively. The concentrations of the three components in rat plasma were determined by LC-MS/MS. DAS 2.0 software was applied to calculate the pharmacokinetic parameters while the SPSS 17.0 software was used for statistical analysis. Significant difference (P ( 0.05) was found between each monomer and the injection on the main pharmacokinetic parameters such as AUC , CL and t1/2. Compared with the injection, the group treated with the syringin has obvious decrease in AUC , and increase in CL while