牵牛化学成分的研究Ⅲ.docVIP

牵牛化学成分的研究Ⅲ 王玉起，李灵娜，华准，王金兰，赵明（齐齐哈尔大学化学与化学工程学院，齐齐哈尔 161006） 摘要目的对化学成分进行研究，从中筛选活性成分牵牛 (Pharbitis purpurea (L.) Voigt )化学成分方法使用重结晶等进行分离纯化，理化性质和光谱分析鉴定结构。结果分离并鉴定了个化合物7-羟基香豆素，β-谷甾醇葡萄糖苷?-谷甾醇 (6)，7?-羟基-β-谷甾醇，?-胡萝卜素 (10)。结论圆叶牵牛 关键词Studies on the chemical constituents of Pharbitis nil (L.) Chiosy Ⅲ WANG Yu-Qi, LI Ling-na, HUA Zhun, Wang Jin-lan, ZHAO Ming (College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, 161006) ABSTRACT: OBJECTIVE The chemical constituents of the aerial part of Pharbitis nil (L.) Chiosy were studied for searching bioactive natural products and compared with Pharbitis purpurea (L.) Voigt. METHODS The constituents were isolated and purified through column chromatography, HPLC and recrystallized and identified on the basis of their physiochemical and spectral data. RESULETS Ten compounds were isolated and identified as N-p-hydroxy-trans-coumaroyltyramine (1), cinnamoyl-tyramine (2), tyramine (3), 7-hydroxycoumarin (4), ?-sitosterol-3-O-?-D-glucoside (5), ?-sitosterol (6), poriferast-5-en-3?,7?-diol (7)，fredelin (8)，?-carotene (10). CONCLUSION The constituents of Pharbitis nil (L.) Chiosy were similar by compareing with Pharbitis purpurea (L.) Voigt. The compounds 7, 8 are isolated from Pharbitis for the first time. The compounds 1-10 are isolated from Pharbitis nil (L.) Choisy for the first time. KEY WORDS: Pharbitis nil (L.) Chiosy; amide; coumarin; friedelane 牵牛为旋花科 (Convolvoulaeae)植物，有圆叶牵牛 (Pharbitis purpurea (L.) Voigt) 和裂叶牵牛 (Pharbitis nil (L.) Choisy) 两种。圆叶牵牛。裂叶牵牛7-羟基香豆素，β-谷甾醇葡萄糖苷?-谷甾醇 (6)，7?-羟基-?-谷甾醇 (7)，木栓烷-3-酮 (8)，?-胡萝卜素 (10)。其中，化合物1~5从圆叶牵牛 x-6显微熔点测定仪（温度未经校正）；Bruker AM-400型核磁共振波谱仪TMS为内标；高效液相色谱仪；薄层色谱硅胶板（烟台化工厂生产）；柱色谱硅胶（200-300目青岛海洋化工厂产品，溶剂为国药集团上海试剂厂。牵牛于200年月采于齐齐哈尔大学校园，经齐齐哈尔大学教授鉴定为Pharbitis (L.) Chiosy。 图1 化合物的结构式 2 提取分离 干燥牵牛㎏，切碎，95%乙醇浸泡，浸泡液经过滤、浓缩00 mL。依次用石油醚、乙酸乙酯、正丁醇萃取，减压蒸馏得到各层浸膏。乙酸乙酯层经硅胶柱色谱分离，3 结构鉴定圆叶牵牛7-羟基香豆素，β-谷甾醇葡萄糖苷mp 135-137 oC。1H NMR (400 MHz, CDCl3)和13C NMR (100 MHz, CDCl3) 与文献[3]对照一致。确定为?-谷甾醇。 化合物7：无色针状结晶 (乙酸乙酯)，m