Effects of alpha particles on zebrafish embryos 24th ICNTS对斑马鱼胚胎的第二十四icntsα粒子的影响.pptVIP

Alpha-particle-induced bystander effects between zebrafish embryos in vivo  E.H.W. Yum1, V.W.Y. Choi1, V.W.T. Li2, S.H. Cheng2, and K.N. Yu1 1Department of Physics and Materials Science, City University of Hong Kong 2Department of Chemistry and Biology, City University of Hong Kong Introduction Objectives: To study whether alpha-particles can induce in vivo bystander effects Identifying the relationship between the alpha-particle absorbed dose and the bystander effect Zebrafish embryos as the model Introduction Radiation-induced Bystander Effect At cell level: Unirradiated cells respond as if they have been irradiated When contact with irradiated cells Or in medium previously holding irradiated cells Introduction Bystander Effect Introduction Bystander effect in vivo are always questioned Introduction Recent researches on bystander effects in vivo Introduction Zebrafish development stages: Introduction Why study alpha particles ? An ionizing radiation with high linear energy transfer (LET) Furthermore, alpha-particle emitters can be easily found in our environment. Introduction Introduction In this project: Irradiation : 1.5 hours after fertilization No repair mechanism at this stage ? can magnify our results for investigation Apoptotic cell staining : 24 hours after fertilization Bladen’s group found it the best time to investigate the no. of apoptotic cell after trying different time stages Methodology Dechorionation of zebrafish embryos Alpha-particle irradiation Bystander signal exposure Apoptotic cell staining Determining the absorbed dose Methodology Dechorionation of zebrafish embryos Range of 5.5 MeV α-particles in water = 42 μm The chorion will absorb all the alpha-particle energies Methodology Methodology Alpha-particle irradiation Methodology Alpha-particle irradiation Methodology PADC films of 16 μm were used polyallyldiglycol-carbonate Repeating unit of PADC: Methodology Methodology Methodolog