多维指纹图谱在中药质量评价中的应用.docVIP

多维指纹图谱在中药质量评价中的应用 　　[摘要] 本文综述了自2000年以来提出多维指纹图谱之后多维指纹图谱技术的发展及其在中药质量控制中的应用。就建立多维指纹图谱的一些联用技术（HPLC-DAD、HPLC-DAD-MS、HPLC-DAD-ELSD、HPLC-DAD-NMR、CE-MS、GC-MS）在多维指纹图谱研究中的优点及应用做一概述，综述了各种色谱及光谱指纹图谱联用的特点，简要阐述了国内外多维指纹图谱的研究在中药质量评价中的应用情况。本文认为多维指纹图谱能够更全面地评价中药的质量。中药指纹图谱的发展必将是多仪器、多测定方式联用的多维指纹图谱。 　　[关键词] 中药；色谱技术；光谱技术；多维指纹图谱 　　[中图分类号] R284.1 [文献标识码] A [文章编号] 1673-7210（2014）08（c）-0164-04 　　Application of multi-dimensional fingerprints in TCM quality evaluation 　　CAO Ling LIU Lili WANG Lianzhi FU Lei 　　College of Pharmacy， Heilongjiang University of Chinese Medicine， Heilongjiang Province， Harbin 150040， China 　　[Abstract] This paper reviews the development of multi-dimensional fingerprint technology and the application of this technology in the quality control of traditional Chinese medicine after multi-dimensional fingerprint was put forward since 2000. The paper summarizes the advantage and application in the study of multi-dimensional fingerprint of some hyphenated techniques， such as HPLC-DAD， HPLC-DAD-MS， HPLC-DAD-ELSD， HPLC-DAD-NMR， CE-MS and GC-MS， which established multi-dimensional fingerprint. The features of the various combination between chromatographic fingerprints with spectroscopic fingerprints were reviewed. The application of multi-dimensional fingerprints in the quality assessment of traditional Chinese medicine at home and abroad is briefly described. This paper considers that multi-dimensional fingerprints can more comprehensively evaluate the quality of traditional Chinese medicine. The development of the fingerprints of traditional Chinese medicines will certainly be a combined multi-dimensional fingerprints with many instruments and various measure manners. 　　[Key words] Traditional Chinese medicine； Chromatographic technique； Spectroscopic technique； Multi-dimensional fingerprint 　　中药指纹图谱是指中药材、提取物或中药制剂等经适当处理后，采用一定的分析手段，得到的能够标示该中药材、提取物或中药制剂特性的共有峰的图谱。从1984年香港中文大学中药研究中心提出了开展中药成分“指纹”鉴定工作的设想以来，我国就开始了指纹图谱的研究[1]。国家药品监督管理局于2000年颁布了《中药注射剂指纹图谱研究的技术要求（暂行）》[2]，要