实验10Arabidopsis叶片总RNA的提取及浓度测定.pptVIP

C Trizol 试剂提取植物组织RNA During sample homogenization or lysis, TRIZOL Reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation, separates the solution into an aqueous phase and an organic phase. RNA remains exclusively in the aqueous phase. After transfer of the aqueous phase, the RNA is recovered by precipitation with isopropyl alcohol. After removal of the aqueous phase, the DNA and proteins in the sample can be recovered by sequential precipitation (2). Precipitation with ethanol yields DNA from