兔自体骨髓干细胞移植对缺血再灌注损伤肾功能的影响作者肖丽佳1.docVIP

兔自体骨髓干细胞移植对缺血再灌注损伤肾功能的影响 作者：肖丽佳1；李亚洁1；余月明2；潘兴华3；曹礼应2；杨勇琴3　　(1南方医科大学南方医院护理部，广东 广州 510515；成都军区昆明总医院2肾脏科；3中心实验科，云南 昆明 650032) 摘要：目的??建立兔肾脏缺血再灌注(I/R)损伤模型，通过肾动脉灌注移植自体骨髓干细胞，观察其对I/R损伤肾功能的影响。方法??分离28只兔的骨髓干细胞，建立肾脏I/R损伤模型后随机均分为移植组和对照组。移植组于肾血流恢复后经肾动脉推注骨髓干细胞悬液，对照组同样方法推注等量生理盐水。分别于I/R损伤前和I/R损伤后第1、3、5、7、14、21、28天于兔耳缘静脉采血，检测血清肌酐(Scr)和尿素氮(BUN)，观察肾功能情况，相同时间点取肾组织行病理学观察。结果?? I/R损伤后第1天和第3天两组动物Scr、BUN均达到最高水平，以后逐渐下降；第7天后移植组Scr及BUN水平逐渐低于对照组，到实验观察结束时的第28天，对照组Scr和BUN分别为(135.6±32.5)μmol/L和(10.9±2.5)mmol/L，移植组Scr和BUN分别为(90.1±11.1)μmol/L和(8.0±1.5)mmol/L，两组间有显著性差异(P0.05)。病理学观察发现，I/R损伤后肾小管上皮细胞变性、坏死、脱落。结论??骨髓干细胞移植能较早的降低肾脏I/R损伤后血清Scr和BUN水平，在一定程度上促进I/R损伤后肾功能修复。 关键词:骨髓干细胞；缺血再灌注；急性肾功能衰竭；移植 中图分类号：R746??文献标识码：A??文章编号：1673-4254(2006)05-0561-03 Effect of autologous bone marrow stem cell transplantation on renal function following renal ischemic-reperfusion in rabbitsXIAO Li-jia1； LI Ya-jie1；YU Yue-ming2； PaN Xing-hua3； CAO Li-ying2；YANG Yong-qin31DePartment of Nursing, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China;2DePartment of Nephrology,3Central Laboratory，Kunming General Hospital of Chengdu Command, Kunming 650032, ChinaAbstract: Objective??To observe the effect of autologous bone marrow stem cell (BMSC) transplantation via the renal artery on renal function recovery following renal ischemic-reperfusion (I/R) injury in rabbits. Methods BMSCs were collected and isolated from rabbits. Twenty-eight rabbits were subjected to renal pedicle clamping for 105 min and randomized subsequently into transplantation group and control group. BMSCs or saline were injected into the kidney via the renal artery, respectively. Before and 1, 3, 5, 7, 14, 21, and 28 days after I/R injury the venous blood was collected to measure the serum levels of SCr and BUN, and the renal tissue was sampled for Pathological observation. Results One and 3 days after I/R injury, serum Cr and BUN levels increased significantly to the highest level in both groups. On the 7th day serum Cr and BUN levels in t