原核生物DNA复制中的酶和蛋白质-2.ppt.ppt

Prepriming involves formation of a complex by sequential association of proteins, leading to the separation of DNA strands. methylation at the origin A membrane-bound inhibitor binds to hemimethylated DNA at the origin, and may function by preventing the binding of DnaA. It is released when the DNA is remethylated. SeqA The complex at oriC can be detected by electron microscopy. Antibodies of dnaA protein HU The protein HU is a general DNA-binding protein in E. coli . Its presence is not absolutely required to initiate replication in vitro, but it stimulates the reaction. HU has the capa