Mycology Lab Methods Austin Community College 真菌学实验室方法奥斯汀社区学院.pptVIP

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Mycology Lab Methods Austin Community College 真菌学实验室方法奥斯汀社区学院.ppt

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Mycology Lab Methods Austin Community College 真菌学实验室方法奥斯汀社区学院

Mycology: Lab Methods Collection of specimens: Skin specimens - clean with 70% alcohol to remove dirt, oil and surface saprophytes. Nails - cleaned same as skin. Usually clipped; need to be finely minced before inoculating to media. Hair - obtained from edge of infected area of scalp; hair can be obtained by plucking, brushing, or with a sticky tape. A Wood’s lamp can be helpful in locating infected areas. Body fluids - normal sterile collection procedures. Mycology: Lab Methods Preparation of specimens for transport: Hair nails sent in a dry envelope, inside proper container. Other specimens are usually sent frozen or on dry ice. Packaging - must meet biohazard regulations. Cultures must be on tubed media (not plates). Inside labeling information: patient ID, specimen source, suspected organism. Outside labeling - WARNING: POTENTIAL PATHOGEN Mycology: Lab Methods Processing of specimen to recover fungus: Skin, nails, hair - direct exam following KOH preparation. Body fluids - CSF - centrifuged; examine sediment microscopically, inoculate media. Pleural fluid, sputum, and bronchial aspiration - specimen must be fresh as saprophytes would overgrow pathogens such as H. capsulatum. Specimens may be refrigerated up to 2 hours. Gastric washings - same as for pleural fluids. Mycology: Lab Methods Processing of specimen to recover fungus: Body fluids - (continued) Genito-urinary specimens - first morning specimen preferred; centrifuge inoculate on media. Blood/bone marrow - inoculate to BHI broth and BHI slant. Wound abscess or drainage - culture anaerobically if actinomycosis is suspected. Tissue specimens - examine for pus, caseous material or granules; mince aseptically, inoculate on media. Mycology: Lab Methods Direct examination of specimens: Direct exam required on any biological material sent to lab for fungus culture. Examine for spores, hyphae, mycelial elements, budding yeast, mycotic granules. Wet mount - good for yeast; examination is done in nat