Venipuncture Blood Collection Micronutrient Initiative静脉穿刺采血微量营养素倡议.pptVIP

Fingerstick Blood Collection into a Microtainer for PNG Universal precautions Finger stick procedure Place all collection materials on top of disposable pad. Open the alcohol swabs, gauze, and bandage. Have all items ready for blood collection. Attach the patient’s labels to the malaria slide and DBS card. Finger stick procedure Finger stick procedure Finger stick procedure Finger stick procedure Finger stick procedure Finger stick procedure Finger stick procedure Finger stick procedure * Assume that all human blood is potentially infectious for HIV, hepatitis, and other infectious agents. Practice universal precautions using gloves, eye protection, and lab coats. Always use sterile, single-use, disposable supplies for sample collection. Put on your powder free gloves. Turn participant’s hand upward. Massage participant’s hand and lower part of the finger to increase blood flow. Scrub the participant’s middle finger or ring finger with an alcohol swab. Dry with gauze. Hold the finger in an upward position and lance the palm side surface of the finger (between the nail and the finger pad) with the lancet. Press firmly on the finger when making the puncture. Doing so will help you to obtain the amount of blood you need. Apply slight pressure to start blood flow. Blot the first drop of blood on a gauze pad and discard in appropriate container. Keep the finger in a downward position and gently massage it to maintain blood flow. Hold the Microtainer? at an angle of 30 degrees below the collection site and use the scoop on the Microtainer? to guide the drop into the vial. Do not scrape the skin. Fill the Microtainer? to 350 - 500 μL level. Cap the Microtainer? and gently invert it 10 times to prevent clots from forming. Apply a sterile adhesive bandage over the puncture site. Place the label on the vial. If the label contains a barcode, the barcode needs to be vertical like a ladder when placed on the vial. If the barcode is not vertical, the laborat