FK1706促进外周神经移植术后大鼠神经轴突再生的研究.docVIP

FK1706促进外周神经移植术后大鼠神经轴突再生的研究 　　【摘要】 目的：探讨FK1706是否能够促进外周神经移植术后大鼠神经轴突再生。方法：20只雄性SD大鼠，行左侧坐骨神经离断、自体桡神经移植术。术后随机分为两组，A组术后当天开始患肢局部肌肉注射FK1706（0.32 mg/kg），持续8周。B组作为对照组不施加干预，仅常规喂养。术后8周，行大鼠左坐骨神经再生有髓神经纤维数及截面积测定、神经电生理检测、左腓肠肌肌湿重测定。结果：A组近端有髓神经纤维数与B组比较差异无统计学意义（P0.05），但远端有髓神经纤维数、远端纤维截面积明显优于B组，差异有统计学意义（P0.01）。A组再生有髓神经纤维的髓鞘厚度（mt）及直径比（d/D）明显优于B组，差异有统计学意义（P0.05）。A组复合运动动作电位（CMAP）、运动神经传导速度（MNCV）及腓肠肌肌湿重明显优于B组，差异有统计学意义（P0.01）。结论：大鼠坐骨神经移植术后应用FK1706具有神经营养作用，可加快神经功能的恢复。 　　【关键词】 FK1706； 营养神经； 自体神经移植； 神经再生 　　【Abstract】 Objective：To investigate whether FK1706 can promote the axon regeneration of peripheral nerve for rats after neural transplantation.Method：20 male SD rats were randomly assigned into two groups after they were performed with left sciatic nerve transection and autologous radial nerve grafting.Group A received FK1706 （0.32 mg/kg） local intramuscular injected once a day on left lower limb for 8 weeks， while Group B as control were routinely fed without special intervention.Electrophysiological studies of left sciatic nerve were performed.The number and area of the regenerated myelinated nerve fiber in cross section pane， and wet muscle weight of the left gastrocnemius muscle were measured，respectively，at 8 weeks after operation.Result：The number of myelinated nerve fiber in proximal end， two groups has no significant difference in statistic （P0.05）， however， in distal end，number of myelinated nerve fiber in Group A was significantly higher than in Group B（P0.01）. The thickness of myelin sheath （mt） of the myelinated nerve fiber， and the ratio that bare axon diameter divided by the total fiber diameter （d/D） were more favorable in Group A than the other， which difference has statistical significance（P0.05）.The wet muscle weight of left gastrocnemius muscle， as well as the compound motor action potential （CMAP） amplitude and motor nerve conduction velocity （MNCV） in Group A，were much better than in Group B，with significant differences （P0.01）.Conclusion：FK1706 has neurotrophy eff