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SectionIII—PrinciplesofBiosafety
Section III—Principles of Biosafety
A fundamental objective of any biosafety program is the containment of potentially
harmful biological agents. The term “containment” is used in describing safe
methods, facilities and equipment for managing infectious materials in the
laboratory environment where they are being handled or maintained. The
purpose of containment is to reduce or eliminate exposure of laboratory workers,
other persons, and the outside environment to potentially hazardous agents.
The use of vaccines may provide an increased level of personal protection.
The risk assessment of the work to be done with a specific agent will determine
the appropriate combination of these elements.
Laboratory Practices and Technique
The most important element of containment is strict adherence to standard
microbiological practices and techniques. Persons working with infectious agents
or potentially infected materials must be aware of potential hazards, and must be
trained and proficient in the practices and techniques required for handling such
material safely. The director or person in charge of the laboratory is responsible
for providing or arranging the appropriate training of personnel.
Each laboratory should develop or adopt a biosafety or operations manual
that identifies the hazards that will or may be encountered, and that specifies
practices and procedures designed to minimize or eliminate exposures to these
hazards. Personnel should be advised of special hazards and should be required
to read and follow the required practices and procedures. A scientist, trained and
knowledgeable in appropriate laboratory techniques, safety procedures, and
hazards associated with handling infectious agents must be responsible for the
conduct of work with any infectious agents or materials. This individual should
consult with biosafety or other health and safety professionals with regard to
risk assessment.
When standard laboratory practi
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