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Easy fabrication of aligned PLLA nanofibers-based 2D scaffolds suitable for cell contact guidance studies.pdf

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Easy fabrication of aligned PLLA nanofibers-based 2D scaffolds suitable for cell contact guidance studies.pdf

Materials Science and Engineering C 62 (2016) 301–306 Contents lists available at ScienceDirect Materials Science and Engineering C journal homepage: /locate/msec Easy fabrication of aligned PLLA nano?bers-based 2D scaffolds suitable for cell contact guidance studies John Mohanraj a, Luca Puzzi a, Ennio Capria b, Stefania Corvaglia b, Loredana Casalis b, Luisa Mestroni c, Orfeo Sbaizero a, Alessandro Fraleoni-Morgera ?a,b,d, a Department of Engineering and Architecture, Univ. of Trieste — V. Valerio 10, 34100 Trieste, Italy b Sincrotrone Trieste SCpA, s.s. 14 km 163,5, 34149 Basovizza, TS, Italy c University of Colorado Denver AMC, 13001 E17th, Aurora, CO 80045, USA d CNR Nano S3, Via Campi 213/A, 41125 Modena, Italy article info Article history: Received 16 July 2015 Received in revised form 3 November 2015 Accepted 18 December 2015 Available online 23 December 2015 Keywords: Scaffold PLLA ASB-SANS HeLa NIH-3T3 Contact guidance abstract An easy, low-cost and fast wet processing-based method named ASB-SANS (Auxiliary Solvent-Based Sublimation-Aided NanoStructuring) has been used to fabricate poly(L-lactic acid) (PLLA) highly ordered and hierarchically organized 2D ?brillar patterns, with ?ber widths between 40 and 500 nm and lengths exceeding tens of microns. A clear contact guidance effect of these nano?brillar scaffolds with respect to HeLa and NIH-3T3 cells growth has been observed, on top of an overall good viability. For NIH-3T3 pronounced elongation of the cells was observed, as well as a remarkable ability of the patterns to guide the extension of pseudopodia. Moreover, SEM imaging revealed ?lopodia stemming from both sides of the pseudopodia and aligned with the secondary PLLA nano?brous structures created by the ASB-SANS procedure. These results validate ASB-SANS as a technique capable to provide biocompatible 2D nano?brillar patterns suitable for studying phenomena of contact guidance (and, more in general, the behavior of cells onto nano?brous scaffolds),