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Solid contact ion sensor with conducting polymer layer copolymerized with the ion-selective membrane for determination of calcium in blood serum.pdf

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Solid contact ion sensor with conducting polymer layer copolymerized with the ion-selective membrane for determination of calcium in blood serum.pdf

Analytica Chimica Acta 943 (2016) 50e57 Contents lists available at ScienceDirect Analytica Chimica Acta journal homepage: /locate/aca Solid contact ion sensor with conducting polymer layer copolymerized with the ion-selective membrane for determination of calcium in blood serum Natalia Abramova a, b, Javier Moral-Vico c, Jordi Soley a, Cristina Ocan~a a, Andrey Bratov a, * a BioMEMS Group, Instituto de Microelectronica de Barcelona, Centro Nacional de Microelectronica (CSIC), Campus U.A.B., 08193, Bellaterra, Barcelona, Spain b Lab. Arti?cial Sensors Syst., ITMO University, Kronverskiy pr. 49, 197101, St. Petersburg, Russia c Department of Chemical, Biological and Environmental Engineering, Escola d’Enginyeria, Universitat Autonoma de Barcelona, 08193, Bellaterra, Barcelona, Spain highlights A new approach of solid contact ion selective electrodes (ISE) fabrication is tested. Conductive polymer layer is copolymerized with ion-selective membrane. The ISE design prevents formation of water layer at the boundary between two polymers. The ISE shows enhanced stability and life-time. The sensor may be used for calcium ion determination in blood serum. article info Article history: Received 2 June 2016 Received in revised form 13 September 2016 Accepted 14 September 2016 Available online 21 September 2016 Keywords: Solid-contact ion selective electrode Conducting polymer Calcium ions Blood serum graphical abstract abstract A new solid contact ion selective electrode with intermediate conducting polymer (CP) layer formed by electropolymerization on a gold electrode of a bifunctional monomer, n-phenyl-ethylenediaminemethacrylamide (NPEDMA), which contains a methacrylamide group attached to aniline, is presented. The conducting polymer was studied by means of optical spectroscopy, cyclic voltammetry and potentiometric measurements. Ca2t-ion-selective membrane based on acrylated urethane polymer was shown to co-polymerize with the CP forming highly adhesive bound