MTT药物敏感试验在指导胃癌及原发肝癌化疗中的作用.pdfVIP

肿瘤防治研究2001年第28卷第 1期 · 37· MTT药物敏感试验在指导胃癌 及原发肝癌化疗中的作用 唐亚辉 ，马 葵 ，韩居然 ，衷淑雯 ，王 卉 摘 要：目的 探讨3一(4，5)一双甲基一2一噻唑 (2，5)一=苯基溴化四氮唑蓝(MTT)药敏试验法 在指导临床胃癌及原发肝癌化疗中的作用。方法 50例胃癌和45倒肝癌，分设对照组。在化疗前 接受MTT药敏试验者，根据药敏结果指导化疗，对照组胃癌23例，肝癌20例 凭经验化疗。结果 在胃癌中MTT敏感组有效率为82．6 (1 9／23)，MTT耐药组和对照组有效率分别为0(0／4)及 47．8 (1】／23)，差异有显著意义{肝癌MTT敏感组有效率为75 (1 5／20)，MTT耐药组和对照 组有效率分别为0(0／5)及4O (8／20)有显著差异(P0．01)。本研究体外药敏和体内疗效总符合 率胃癌和肝癌分别为85．2 (1 9+4／27)和80 (15+5125)。结论 MTT体外药敏试验在指导临 床胃癌及原发肝癌的化疗中，具有满意的效果。 关键词：胃癌；肝癌；药敏试验；MTT法 中图分类号：R73—36 1 文就标识码：A 文章编号：1000—8578(2001)O1—0037—02 Application of MTT to Stomach and Liver Carcinoma in Vitro Drug—sensitivity Testing TANG Ya—hui，MA Kui，HAN Ju—ran，et al The nevy Genery fIospitol，Haemotology Dept，Beijing 100037，China Abstract：Objective The predictive value of in vitro MTT assay for chemotherapy to stomach carcino— ma and liver cancer patients was studied．Methods Of the 50 stomach carcinoma patients，27 had had MTT assay before chemotherapy．According to the result of the MTT assay，23 patients in MTT sensitive group received chemotherapy．The other 4 patients in MTT resistant group and 23 patients in the control group were given chemotheraphy according to clinician exparienee，the same liver cancer 25 eases in MTT，5 in re— sistant group and 20 in the control group．Results The response rate in MTT sensitive group was 82．6 in stomach carcinoma，75 in liver cancer，respectively．There was statistieally significant difference in the sensitive group as compared in the resistant group and in control group．Between in vitro and in vivo，the overall coincident rate was 85．2 (1 9+4／27)and 80 (15+5／25)，respectively．ConclusionThe in vitro MTT assay can predict chemotherapy for stomach and liver carcinoma giving satisfa