天气对青蒿发性质的影响学士学位思考.docVIP

本科生毕业论文 天气对青蒿挥发性物质的影响 Student ID: 071040110 BACHELORS THESIS OF HUBEI UNIVERSITY FOR NATIONALTIES The effect of weather on the contents and the kinds of volatile substances released from the leaves of Artemisia annua L. Faculty Name: School of Biological Science and technology Major: Biological engineering Stu-Name: Huang Hui Mentor: Zhou Yi-feng Enshi China May 6th, 2014 郑重声明 本人郑重声明所呈交的学位论文《天气对青蒿挥发性物质影响情况的分析》是我在导师 周毅峰 的指导下独立进行研究工作所取得的研究成果。除文中已经注明引用的内容外，论文中不包含其他个人已经发表或撰写过的研究成果，也不包含为获得湖北民族学院或其它教育机构的学位或证书而使用过的材料。对本文的研究做出重要贡献的个人和集体，均已在文中以明确的方式进行了说明。 本声明的法律结果由本人独自承担。 作者签名： 年 月 日  天气对青蒿挥发性物质的影响 黄 惠 （湖北民族学院生物科学与技术学院，湖北恩施，445000） 摘 要：为了解不同天气环境下青蒿释放的挥发性成分和含量的变化，利用顶空固相微萃取-气相色谱-质谱联用仪（SPME-GC/MS）测定青蒿在不同天气下的挥发性成分。结果表明：1）青蒿的挥发性物质在不同的天气其种类不同：(1R)-(+)-α蒎烯、水芹烯、环氧法尼烯只存在雨天的样品中检测出来，晴天没有；而π-薰衣草乙酯、顺-2-蒈烯-4-醇只在晴天的青蒿样品中检测出来，雨天没有；2）青蒿的挥发性物质在晴天和雨天的百分含量不同：青蒿的挥发性单萜及其衍生物的相对百分含量大多雨天高于晴天；青蒿挥发性倍半萜及其衍生物的相对百分含量大多晴天高于雨天。 关键词：青蒿；顶空固相微萃取-气相色谱-质谱联用（SPME-GC/MS）；挥发性物质 Abstract:In order to know the changes of volatile components and contents in Artemisia annua L which are released during different weathers,we determine the volatile components of Artemisia annua L in different weather conditions by using SPME-GC/MS.The results show that:1)The kinds of volatile components in Artemisia annua L are distinct in different weather conditions:(1R)-(+)-alpha-Pinene、phellandrene and epoxy farnesene are only detected in the samples of rainy days,while π - Lavender ethyl alcohol and CIS -2- CARENE -4- alcohol are merely detected in the samples of sunny days;2)The percentages of volatile substances in Artemisia annua L during sunny days are different with that of the rainy days:the percentages of volatile monoterpene and its derivatives of Artemisia annua L in rainy days are mostly higher than that of the sunny days; the percentage of volatile sesquiterpenes and its derivatives of Artemisia annua L in sunny days are mostly higher than that of the rainy da