结合微孔膜乳化法和复乳溶剂蒸发法制备粒径均一聚乳酸微囊的研究.pdfVIP

M020 结合微孔膜乳化法和复乳溶剂蒸发法制备粒径均一 聚乳酸微囊的研究 刘荣，孟凡涛，马光辉* ( 中国科学院过程工程研究所生化工程国家重点实验室，北京，100080) * 通讯联系人:Tel:010Fax:010 E-mail:ghma@home.ipe.ac.cn. 摘 要 本文采用一种新型的乳化方法——微孔膜乳化法，首次将其与复乳溶剂蒸发法相结合， 制备粒径均一的高分子微囊。结果表明，将油相密度调至 1mg/ml 并采用2.5 ％ Arlacel 83 作为油 相乳化剂可使初乳稳定24h 以上。随后本文就外水相中pH 值对药物包埋率的影响进行了研究并 与机械搅拌法进行了对比，结果表明，采用膜乳化法能大大提高药物的包埋率并能得到粒径均一 的聚乳酸微囊，最终药物包埋率可达到92.20 ％，聚乳酸微囊的粒径分布率达到14.7 ％。 关键词 微孔膜乳化法 复乳溶剂蒸发法 聚乳酸 药物包埋率 Preparation of Uniform-sized PLA microcapsules combining the membrane emulsification technique and multiple emulsion-solvent evaporation method Rong Liu, Fantao Meng, GuangHui Ma （State Key La boratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O.Box 353, Beijing 100080, China） Abstract Relatively uniform biodegradable poly (lactide) (PLA) microcapsules were successfully prepared by combining the membrane emulsification technique and multiple emulsion-solvent evaporation method. It was found that when the density difference of the internal water phase and oil phase was reduced to nearly zero and Arlacel 83 was used as the oil emulsifier, the primary emulsion was maintained stable and the phase separation was not observed within 24h. The effect of pH values in the external water phase on the drug encapsulation efficiency was investigated. The characteristics of microcapsules prepared with membrane emulsification technique and stirring method were compared, it was found that the size was more uniform when the microcapsules were prepared by membrane emulsification technique and the highest drug encapsulation efficiency of 92.20% was obtained. Keywords membrane emulsification; Multiple emulsion-solvent eva