紫砂芸豆花色苷的超声微波协同提取研究及其组成分析（.doc

紫砂芸豆花色苷的超声/微波协同提取研究及其组成分析( 李良玉1,2 宋大巍3 刁静静1 贾鹏禹1 张丽萍1,3 （1黑龙江八一农垦大学国家杂粮工程技术研究中心，大庆 163319；2 东北林业大学林学院，哈尔滨，150040；3黑龙江八一农垦大学食品学院，大庆 163319) 摘要：以东北紫砂芸豆为原料，采用单因素试验和响应面优化试验研究超声/微波协同提取紫砂芸豆中花色苷的工艺参数，并采用液质连用色谱法对提取物进行不同浓度乙醇洗脱，进行组分分析。结果表明，超声/微波协同提取紫砂芸豆花色苷的有效工艺参数为：微波功率251 W，液料比13.7 mL/g，提取时间24.9min。不同浓度乙醇洗脱液的色谱分析发现，提取物中主要有5种花色苷物质，其中天竺葵素-3-葡萄糖苷、芍药素-3-葡萄糖苷含量较高，且主要集中在40%乙醇洗脱液中。本研究为大孔树脂纯化紫砂芸豆花色苷提供了参考，为紫砂芸豆花色苷的提取加工及工业应用提供依据。 关键词：紫砂芸豆 花色苷 超声/微波协同提取 组成分析 中图分类号: TS214.9 文献标识码: A 文章编号: Research on ultrasonic/microwave synergistic extraction of anthocyanins from purple kidney bean and structure identification Li Liang-yu1 Song Da-wei2 Diao Jing-jing1 Jia peng-yu1 Zhang Li-ping1,2* (1 National Coarse Cereals Engineering Research Center, Heilongjiang Bayi Agricultural University, Daqing 163319；2 College of Forestry, Northeast Forestry University, Harbin 150040；3 College of Food, Heilongjiang Bayi Agricultural University, Daqing 163319) Abstract：In this paper, purple kidney bean varieties from northeast of China was taken as the raw material, the ultrasonic/microwave synergistic extraction process of anthocyanins from purple bean were studied, and the structure identified. As the results indicated , the optimum ultrasonic/microwave synergistic extraction process of anthocyanins from purple kidney bean were as following: The microwave power 251 W, the liquid to solid ratio 13.7 mL/g, extraction time 24.9min. In this study, the species and composition of anthocyanin from purple bean were identified. There are mainly five kinds of anthocyanins substances, in which pelargonidin 3-glucoside, peony-3-glucoside were higher by of different concentration ethanol elution liquid quality analysis, pelargonidin3-glucoside, peony prime3-glucoside mainly concentrated in 40% ethanol eluent. The research may provide reference for anthocyanin purification with macroporous resin, and as theory base for anthocyanin process and its industrial application. Keywords: purple kidney bean, anthocyanin