降钙素对乳鼠成骨细胞增殖及IGFI表达的影响.pdfVIP

???????? ???λ???? ?????????????????????IGF-I ??????? ???????????? ?????λ?????? ???????? ?????????? ? 2011-05-15 ???????????λ???? I ????????????????????? IGF-I ??????? ? ? ??????????????????????(calcitonin??CT)??????????????????????? ???????????????????????????????????????????-I??Insulin-like Growth Factor-I??IGF-I??????????????????????????????????????????????? ??????? ??????????? 24h SD ????-?????????????????????????(osteoblast??OB)???? ?????????????????????????????????????????????????ALP?????????????? ??????????????????????????????ò?????(1??10 -12 ??1??10 - 8 mol/ L)?? CT ?? ??????? MTT ??????????????????? OB ???????Gomori ??-?????????????(ALP) ???? RT-PCR ??????????????????????-I (IGF-I) mRNA ???? ?????????????????????????????MTT ???????????????????? 1 ??10 - 12 mol/ L ???????? OB ????????? CT ???????????????????????????????????Gomori ?? -???????????????????????????? OB ?????????????? RT-PCR ??????????IGF-I mRNA ??????????????????????????????????????????????????????? IGF-ImRNA ????????????? ???????????? CT ???????????????????????????CT ??? IGF-I ????????? ????????????? ???????????? ?????? ??? ??????????????? ???????????λ???? II The Influence of Different Concentrations of salmon calcitonin act on the expression of IGF-I and the proliferation of suckling mice osteoblasts Abstract Objectives:Through observe the role of salmon calcitonin (CT) on osteoblasts in vitro culture rat, we are trying to have a preliminary study of the influence of salmon calcitonin on the proliferation of osteoblasts and the expression of IGF-I, this way could provide a theoretical basis of promoting bone repair and regeneration on clinical treatment. Methods:Take the calvarias of 24h SD neonatal rats, with enzyme digestion of osteoblast cells, primary osteoblasts are obtained by enzymatic digestion, cultured and identified osteoblasts, with different concentrations of calcitonin (1 ??10 - 12??1??10 - 8 m